4bj8

Zebavidin

spinqualitylabelsall models 4bj8, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4bj8 is a 16 chain structure with sequence from Brachidanio rerio. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: BTN, GOL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The avidin protein family members are well known for their high affinity towards D-biotin and high structural stability. These properties make avidins valuable tools for a wide range of biotechnology applications. We have identified a new member of the avidin family in the zebrafish (Danio rerio) genome, hereafter called zebavidin. The protein is highly expressed in the gonads of both male and female zebrafish and in the gills of male fish, but our data suggest that zebavidin is not crucial for the developing embryo. Biophysical and structural characterisation of zebavidin revealed distinct properties not found in any previously characterised avidins. Gel filtration chromatography and native mass spectrometry suggest that the protein forms dimers in the absence of biotin at low ionic strength, but assembles into tetramers upon binding biotin. Ligand binding was analysed using radioactive and fluorescently labelled biotin and isothermal titration calorimetry. Moreover, the crystal structure of zebavidin in complex with biotin was solved at 2.4 A resolution and unveiled unique ligand binding and subunit interface architectures; the atomic-level details support our physicochemical observations. Zebavidin - an avidin-like protein from zebrafish.,Taskinen B, Zmurko J, Ojanen M, Kukkurainen S, Parthiban M, Maatta JA, Leppiniemi J, Janis J, Parikka M, Turpeinen H, Ramet M, Pesu M, Johnson MS, Kulomaa MS, Airenne TT, Hytonen VP PLoS One. 2013 Oct 24;8(10):e77207. doi: 10.1371/journal.pone.0077207. PMID:24204770 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Avidin

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