6e3a: Difference between revisions
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==tRNA 2'-phosphotransferase== | |||
<StructureSection load='6e3a' size='340' side='right'caption='[[6e3a]], [[Resolution|resolution]] 1.40Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6e3a]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E3A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E3A FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=HQG:[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]+[(2~{R},3~{S},4~{R},5~{R})-3,4-bis(oxidanyl)-5-phosphonooxy-oxolan-2-yl]methyl+hydrogen+phosphate'>HQG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6e3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e3a OCA], [http://pdbe.org/6e3a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e3a RCSB], [http://www.ebi.ac.uk/pdbsum/6e3a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e3a ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/KPTA_CLOTH KPTA_CLOTH]] Removes the 2'-phosphate from RNA via an intermediate in which the phosphate is ADP-ribosylated by NAD followed by a presumed transesterification to release the RNA and generate ADP-ribose 1''-2''-cyclic phosphate (APPR>P). May function as an ADP-ribosylase. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Tpt1 is an essential agent of fungal tRNA splicing that removes the 2'-PO4 at the splice junction generated by fungal tRNA ligase. Tpt1 catalyzes a unique two-step reaction whereby the 2'-PO4 attacks NAD(+) to form an RNA-2'-phospho-ADP-ribosyl intermediate that undergoes transesterification to yield 2'-OH RNA and ADP-ribose-1'',2''-cyclic phosphate products. Because Tpt1 is inessential in exemplary bacterial and mammalian taxa, Tpt1 is seen as an attractive antifungal target. Here we report a 1.4 A crystal structure of Tpt1 in a product-mimetic complex with ADP-ribose-1''-phosphate in the NAD(+) site and pAp in the RNA site. The structure reveals how Tpt1 recognizes a 2'-PO4 RNA splice junction and the mechanism of RNA phospho-ADP-ribosylation. This study also provides evidence that a bacterium has an endogenous phosphorylated substrate with which Tpt1 reacts. | |||
Structure of tRNA splicing enzyme Tpt1 illuminates the mechanism of RNA 2'-PO4 recognition and ADP-ribosylation.,Banerjee A, Munir A, Abdullahu L, Damha MJ, Goldgur Y, Shuman S Nat Commun. 2019 Jan 15;10(1):218. doi: 10.1038/s41467-018-08211-9. PMID:30644400<ref>PMID:30644400</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6e3a" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Banerjee, A]] | |||
[[Category: Goldgur, Y]] | [[Category: Goldgur, Y]] | ||
[[Category: Shuman, S]] | [[Category: Shuman, S]] | ||
[[Category: Kpta]] | |||
[[Category: Tpt1]] | |||
[[Category: Transferase]] | |||
[[Category: Trpt1]] | |||
