4c9c

Crystal Structure of the Strawberry Pathogenesis-Related 10 (PR-10) Fra a 1E protein (Form A)

spinqualitylabelsall models 4c9c, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4c9c is a 2 chain structure with sequence from Fraan. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GOL, SO4 Related: 4c94 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Pathogenesis-related 10 (PR-10) proteins are involved in many aspects of plant biology but their molecular function is still unclear. They are related by sequence and structural homology to mammalian lipid transport and plant abscisic acid receptor proteins, and are predicted to have cavities for ligand binding. Recently, three new members of the PR-10 family, the Fra a proteins, have been identified in strawberry, where they are required for the activity of the flavonoid biosynthesis pathway, which is essential for the development of color and flavor in fruits. Here we show that Fra a proteins bind natural flavonoids with different selectivity and affinities in the low muM range. The structural analysis of Fra a 1 E and a Fra a 3-catechin complex indicates that loops L3, L5 and L7, surrounding the ligand-binding cavity show significant flexibility in the apo forms, but close over the ligand in the Fra a 3-catechin complex. Our findings provide mechanistic insight on the function of Fra a proteins and suggest that PR-10 proteins, which are widespread in plants may play a role in the control of secondary metabolic pathways by binding to metabolic intermediates. The Strawberry Pathogenesis-Related 10 (PR-10) Fra a proteins control flavonoid biosynthesis by binding to metabolic intermediates.,Casanal A, Zander U, Munoz C, Dupeux F, Luque I, Botella MA, Schwab W, Valpuesta V, Marquez JA J Biol Chem. 2013 Oct 16. PMID:24133217 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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