4hhj

Dengue serotype 3 RNA-dependent RNA polymerase

spinqualitylabelsall models 4hhj, resolution 1.79Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4hhj is a 1 chain structure with sequence from Dengue virus 3. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: P6G, PEG, ZN Related: 3vws Activity: RNA-directed RNA polymerase, with EC number 2.7.7.48 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [Q6DLV0_9FLAV] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS000336_004_099774] Publication Abstract from PubMed We report a highly reproducible method to crystallize the RNA-dependent RNA polymerase (RdRp) domain of dengue virus serotype-3 (DENV-3), allowing structure refinement to 1.79 A resolution and revealing amino acids not seen previously. We also present a DENV-3 polymerase/inhibitor co-crystal structure at 2.1 A resolution. The inhibitor binds to the RdRp as a dimer and causes conformational changes in the protein. The improved crystallization conditions and new structural information should accelerate structure-based drug discovery. Conformational flexibility of the dengue virus RNA-dependent RNA polymerase revealed by a complex with an inhibitor.,Noble CG, Lim SP, Chen YL, Liew CW, Yap L, Lescar J, Shi PY J Virol. 2013 Feb 13. PMID:23408636 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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