4fs5

Crystal structure of the Z-DNA hexamer CGCGCG at 500 mM MgCl2

spinqualitylabelsall models 4fs5, resolution 1.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4fs5 is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CL, MG Related: 4fs6 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed In this study, crystals of Z-DNA hexamer d(CGCGCG) complexed with MgCl2 and CaCl2 were obtained in the presence of high concentrations of alkaline earth salts (500mM) using a temperature control technique, and their crystal structures were determined at 1.3A resolution. Mg2+ and Ca2+ cations in these structures tend to interact directly with phosphate groups of Z-DNA duplexes; however, they tend to form water-mediated interactions with Z-DNA in the presence of lower concentrations of alkaline earth salts. In these crystals, a DNA duplex was laid along its c-axis and interacted with its 6 neighboring DNA duplexes through coordination bonds of PO...(Mg2+ or Ca2+)...OP. A symmetrical hexagonal Z-DNA duplex assembly model may explain DNA condensation caused by alkaline earth salts. These structures offer insights into the functions of alkaline earth cations essential to the structures and assembly of Z-DNA duplexes. Direct interactions between Z-DNA and alkaline earth cations, discovered in the presence of high concentrations of MgCl and CaCl,Chatake T, Sunami T J Inorg Biochem. 2013 Mar 19;124C:15-25. doi: 10.1016/j.jinorgbio.2013.03.004. PMID:23578858 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Z-DNA

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