6dyx: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 6dyx is ~~ON HOLD until Paper~~ Publication

==Structure of VHH R419 isolated from a pre-immune phage display library==

== Structural highlights ==

<table><tr><td colspan='2'>[[6dyx]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DYX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DYX FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dyx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dyx OCA], [http://pdbe.org/6dyx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dyx RCSB], [http://www.ebi.ac.uk/pdbsum/6dyx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dyx ProSAT]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

OBJECTIVE: To determine the X-ray structure and biophysical properties of a Camelid VHH isolated from a naive phage display library. RESULTS: Single domain antibodies (VHH) derived from the unique immune system of the Camelidae family have gained traction as useful tools for biotechnology as well as a source of potentially novel therapeutics. Here we report the structure and biophysical characterization of a VHH originally isolated from a naive camelid phage display library. VHH R419 has a melting temperate of 66 degrees C and was found to be a monomer in solution. The protein crystallized in space group P6522 and the structure was solved by molecular replacement to a resolution of 1.5 A. The structure revealed a flat paratope with CDR loops that could be classified into existing canonical loop structures. A combination of high expression yield, stability and rapid crystallization might make R419 into a candidate scaffold for CDR grafting and homology modeling.

~~Authors~~: ~~Brooks, C~~.L.

Structure of a VHH isolated from a naive phage display library.,White B, Huh I, Brooks CL BMC Res Notes. 2019 Mar 19;12(1):154. doi: 10.1186/s13104-019-4197-0. PMID:30890176<ref>PMID:30890176</ref>

~~Description: Structure~~ of ~~VHH R419 isolated from a pre~~-~~immune phage display library~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

[[Category: ~~Unreleased~~ Structures]]

</div>

[[Category: Brooks, C.L]]

== References ==

__TOC__

</StructureSection>

[[Category: Large Structures]]

[[Category: Brooks, C L]]

[[Category: Immune system]]

[[Category: Nanobody vhh single domain antibody]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 6dyx is ON HOLD  until Paper Publication
+==Structure of VHH R419 isolated from a pre-immune phage display library==
+<StructureSection load='6dyx' size='340' side='right'caption='[[6dyx]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6dyx]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DYX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DYX FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dyx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dyx OCA], [http://pdbe.org/6dyx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dyx RCSB], [http://www.ebi.ac.uk/pdbsum/6dyx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dyx ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+OBJECTIVE: To determine the X-ray structure and biophysical properties of a Camelid VHH isolated from a naive phage display library. RESULTS: Single domain antibodies (VHH) derived from the unique immune system of the Camelidae family have gained traction as useful tools for biotechnology as well as a source of potentially novel therapeutics. Here we report the structure and biophysical characterization of a VHH originally isolated from a naive camelid phage display library. VHH R419 has a melting temperate of 66 degrees C and was found to be a monomer in solution. The protein crystallized in space group P6522 and the structure was solved by molecular replacement to a resolution of 1.5 A. The structure revealed a flat paratope with CDR loops that could be classified into existing canonical loop structures. A combination of high expression yield, stability and rapid crystallization might make R419 into a candidate scaffold for CDR grafting and homology modeling.
-Authors: Brooks, C.L.
+Structure of a VHH isolated from a naive phage display library.,White B, Huh I, Brooks CL BMC Res Notes. 2019 Mar 19;12(1):154. doi: 10.1186/s13104-019-4197-0. PMID:30890176<ref>PMID:30890176</ref>
-Description: Structure of VHH R419 isolated from a pre-immune phage display library
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Brooks, C.L]]
+<div class="pdbe-citations 6dyx" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Brooks, C L]]
+[[Category: Immune system]]
+[[Category: Nanobody vhh single domain antibody]]