6m7m: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 6m7m is ~~ON HOLD until Paper~~ Publication

==rac-GSTSTA from degenerate octameric repeats in InaZ, residues 707-712==

== Structural highlights ==

<table><tr><td colspan='2'>[[6m7m]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M7M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6M7M FirstGlance]. <br>

</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6eex|6eex]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6m7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m7m OCA], [http://pdbe.org/6m7m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6m7m RCSB], [http://www.ebi.ac.uk/pdbsum/6m7m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6m7m ProSAT]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

The ice-nucleation protein InaZ from Pseudomonas syringae contains a large number of degenerate repeats that span more than a quarter of its sequence and include the segment GSTSTA. Ab initio structures of this repeat segment, resolved to 1.1 A by microfocus X-ray crystallography and to 0.9 A by the cryo-EM method MicroED, were determined from both racemic and homochiral crystals. The benefits of racemic protein crystals for structure determination by MicroED were evaluated and it was confirmed that the phase restriction introduced by crystal centrosymmetry increases the number of successful trials during the ab initio phasing of the electron diffraction data. Both homochiral and racemic GSTSTA form amyloid-like protofibrils with labile, corrugated antiparallel beta-sheets that mate face to back. The racemic GSTSTA protofibril represents a new class of amyloid assembly in which all-left-handed sheets mate with their all-right-handed counterparts. This determination of racemic amyloid assemblies by MicroED reveals complex amyloid architectures and illustrates the racemic advantage in macromolecular crystallography, now with submicrometre-sized crystals.

~~Authors:~~ Zee~~, C.~~, Glynn, C., Gallagher-Jones, M., Miao, J., Santiago~~, C.G.~~, Cascio, D., Gonen, T., Sawaya, M.R., ~~Rodriguez, J.A~~.

Homochiral and racemic MicroED structures of a peptide repeat from the ice-nucleation protein InaZ.,Zee CT, Glynn C, Gallagher-Jones M, Miao J, Santiago CG, Cascio D, Gonen T, Sawaya MR, Rodriguez JA IUCrJ. 2019 Jan 24;6(Pt 2):197-205. doi: 10.1107/S2052252518017621. eCollection, 2019 Mar 1. PMID:30867917<ref>PMID:30867917</ref>

~~Description~~: ~~rac~~-~~GSTSTA from degenerate octameric repeats in InaZ~~, ~~residues 707-712~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

[[Category: ~~Unreleased Structures~~]]

</div>

== References ==

__TOC__

</StructureSection>

[[Category: Large Structures]]

[[Category: Cascio, D]]

[[Category: Gallagher-Jones, M]]

[[Category: Glynn, C]]

[[Category: Gonen, T]]

[[Category: Rodriguez, J.A]]

[[Category: Miao, J]]

[[Category: ~~Glynn~~, C]]

[[Category: Rodriguez, J A]]

[[Category: Santiago, C G]]

[[Category: Sawaya, M R]]

[[Category: Zee, C]]

[[Category: ~~Santiago, C.G~~]]

[[Category: Amyloid]]

[[Category: ~~Miao, J~~]]

[[Category: Ice nucleation]]

[[Category: ~~Sawaya, M.R~~]]

[[Category: Inaz]]

[[Category: ~~Cascio, D~~]]

[[Category: Microed]]

[[Category: ~~Gallagher-Jones, M~~]]

[[Category: Protein fibril]]

[[Category: Pseudomonas syringae]]

[[Category: Racemic]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 6m7m is ON HOLD  until Paper Publication
+==rac-GSTSTA from degenerate octameric repeats in InaZ, residues 707-712==
+<StructureSection load='6m7m' size='340' side='right'caption='[[6m7m]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6m7m]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M7M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6M7M FirstGlance]. <br>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6eex|6eex]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6m7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m7m OCA], [http://pdbe.org/6m7m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6m7m RCSB], [http://www.ebi.ac.uk/pdbsum/6m7m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6m7m ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The ice-nucleation protein InaZ from Pseudomonas syringae contains a large number of degenerate repeats that span more than a quarter of its sequence and include the segment GSTSTA. Ab initio structures of this repeat segment, resolved to 1.1 A by microfocus X-ray crystallography and to 0.9 A by the cryo-EM method MicroED, were determined from both racemic and homochiral crystals. The benefits of racemic protein crystals for structure determination by MicroED were evaluated and it was confirmed that the phase restriction introduced by crystal centrosymmetry increases the number of successful trials during the ab initio phasing of the electron diffraction data. Both homochiral and racemic GSTSTA form amyloid-like protofibrils with labile, corrugated antiparallel beta-sheets that mate face to back. The racemic GSTSTA protofibril represents a new class of amyloid assembly in which all-left-handed sheets mate with their all-right-handed counterparts. This determination of racemic amyloid assemblies by MicroED reveals complex amyloid architectures and illustrates the racemic advantage in macromolecular crystallography, now with submicrometre-sized crystals.
-Authors: Zee, C., Glynn, C., Gallagher-Jones, M., Miao, J., Santiago, C.G., Cascio, D., Gonen, T., Sawaya, M.R., Rodriguez, J.A.
+Homochiral and racemic MicroED structures of a peptide repeat from the ice-nucleation protein InaZ.,Zee CT, Glynn C, Gallagher-Jones M, Miao J, Santiago CG, Cascio D, Gonen T, Sawaya MR, Rodriguez JA IUCrJ. 2019 Jan 24;6(Pt 2):197-205. doi: 10.1107/S2052252518017621. eCollection, 2019 Mar 1. PMID:30867917<ref>PMID:30867917</ref>
-Description: rac-GSTSTA from degenerate octameric repeats in InaZ, residues 707-712
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 6m7m" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Cascio, D]]
+[[Category: Gallagher-Jones, M]]
+[[Category: Glynn, C]]
 [[Category: Gonen, T]]
-[[Category: Rodriguez, J.A]]
+[[Category: Miao, J]]
-[[Category: Glynn, C]]
+[[Category: Rodriguez, J A]]
+[[Category: Santiago, C G]]
+[[Category: Sawaya, M R]]
 [[Category: Zee, C]]
-[[Category: Santiago, C.G]]
+[[Category: Amyloid]]
-[[Category: Miao, J]]
+[[Category: Ice nucleation]]
-[[Category: Sawaya, M.R]]
+[[Category: Inaz]]
-[[Category: Cascio, D]]
+[[Category: Microed]]
-[[Category: Gallagher-Jones, M]]
+[[Category: Protein fibril]]
+[[Category: Pseudomonas syringae]]
+[[Category: Racemic]]