5xbi: Difference between revisions
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==The structure of BrlR-C domain bound to 3-amino-2-phenazino(a pyocyanin analog)== | ==The structure of BrlR-C domain bound to 3-amino-2-phenazino(a pyocyanin analog)== | ||
<StructureSection load='5xbi' size='340' side='right' caption='[[5xbi]], [[Resolution|resolution]] 1.40Å' scene=''> | <StructureSection load='5xbi' size='340' side='right'caption='[[5xbi]], [[Resolution|resolution]] 1.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5xbi]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XBI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XBI FirstGlance]. <br> | <table><tr><td colspan='2'>[[5xbi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XBI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XBI FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=81U:3-azanylphenazin-2-ol'>81U</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=81U:3-azanylphenazin-2-ol'>81U</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PA4878 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xbi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xbi OCA], [http://pdbe.org/5xbi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xbi RCSB], [http://www.ebi.ac.uk/pdbsum/5xbi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xbi ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xbi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xbi OCA], [http://pdbe.org/5xbi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xbi RCSB], [http://www.ebi.ac.uk/pdbsum/5xbi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xbi ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The virulence factor pyocyanin and the intracellular second messenger cyclic diguanylate monophosphate (c-di-GMP) play key roles in regulating biofilm formation and multi-drug efflux pump expression in Pseudomonas aeruginosa. However, the crosstalk between these two signaling pathways remains unclear. Here we show that BrlR (PA4878), previously identified as a c-di-GMP responsive transcriptional regulator, acts also as a receptor for pyocyanin. Crystal structures of free BrlR and c-di-GMP-bound BrlR reveal that the DNA-binding domain of BrlR contains two separate c-di-GMP binding sites, both of which are involved in promoting brlR expression. In addition, we identify a pyocyanin-binding site on the C-terminal multidrug-binding domain based on the structure of the BrlR-C domain in complex with a pyocyanin analog. Biochemical analysis indicates that pyocyanin enhances BrlR-DNA binding and brlR expression in a concentration-dependent manner. | |||
BrlR from Pseudomonas aeruginosa is a receptor for both cyclic di-GMP and pyocyanin.,Wang F, He Q, Yin J, Xu S, Hu W, Gu L Nat Commun. 2018 Jul 2;9(1):2563. doi: 10.1038/s41467-018-05004-y. PMID:29967320<ref>PMID:29967320</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 5xbi" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Pseae]] | |||
[[Category: Gu, L]] | [[Category: Gu, L]] | ||
[[Category: Qing, H]] | [[Category: Qing, H]] | ||
