4m6e

The high resolution structure of tyrocidine A reveals an amphipathic dimer

spinqualitylabelsall models 4m6e, resolution 0.95Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4m6e is a 1 chain structure with sequence from Bacillus brevis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: MOH, MPD NonStd Res: DPN, ORN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Tyrocidine A, one of the first antibiotics ever to be discovered, is a cyclic decapeptide that binds to membranes of target bacteria, disrupting their integrity. It is active against a broad spectrum of Gram-positive organisms, and has recently engendered interest as a potential scaffold for the development of new drugs to combat antibiotic-resistant pathogens. We present here the X-ray crystal structure of tyrocidine A at a resolution of 0.95A. The structure reveals that tyrocidine forms an intimate and highly amphipathic homodimer made up of four beta strands that associate into a single, highly curved antiparallel beta sheet. We used surface plasmon resonance and potassium efflux assays to demonstrate that tyrocidine binds tightly to mimetics of bacterial membranes with an apparent dissociation constant (KD) of 10muM, and efficiently permeabilizes bacterial cells at concentrations equal to and below the KD. Using variant forms of tyrocidine in which the fluorescent probe p-cyano-phenylalanine had been inserted on either the polar or apolar face of the molecule, we performed fluorescence quenching experiments, using both water-soluble and membrane-embedded quenchers. The quenching results, together with the structure, strongly support a membrane association model in which the convex, apolar face of tyrocidine's beta sheet is oriented toward the membrane interior, while the concave, polar face is presented to the aqueous phase. The high resolution structure of tyrocidine A reveals an amphipathic dimer.,Loll PJ, Upton EC, Nahoum V, Economou NJ, Cocklin S Biochim Biophys Acta. 2014 May;1838(5):1199-207. doi:, 10.1016/j.bbamem.2014.01.033. Epub 2014 Feb 11. PMID:24530898 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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