2n85: Difference between revisions

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==NMR structure of OtTx1a - AMP in DPC micelles==

== Structural highlights ==

<table><tr><td colspan='2'>[[2n85]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N85 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N85 FirstGlance]. <br>

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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n85 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n85 OCA], [http://pdbe.org/2n85 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n85 RCSB], [http://www.ebi.ac.uk/pdbsum/2n85 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2n85 ProSAT]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

We have recently demonstrated that a common phenomenon in evolution of spider venom composition is the emergence of so-called modular toxins consisting of two domains, each corresponding to a "usual" single-domain toxin. In this article, we describe the structure of two domains that build up a modular toxin named spiderine or OtTx1a from the venom of Oxyopes takobius. Both domains were investigated by solution NMR in water and detergent micelles used to mimic membrane environment. The N-terminal spiderine domain OtTx1a-AMP (41 amino acid residues) contains no cysteines. It is disordered in aqueous solution but in micelles, it assumes a stable amphiphilic structure consisting of two alpha-helices separated by a flexible linker. On the contrary, the C-terminal domain OtTx1a-ICK (59 residues) is a disulfide-rich polypeptide reticulated by five S-S bridges. It presents a stable structure in water and its core is the inhibitor cystine knot (ICK) or knottin motif that is common among single-domain neurotoxins. OtTx1a-ICK structure is the first knottin with five disulfide bridges and it represents a good reference for the whole oxytoxin family. The affinity of both domains to membranes was measured with NMR using titration by liposome suspensions. In agreement with biological tests, OtTx1a-AMP was found to show high membrane affinity explaining its potent antimicrobial properties.

Modular toxin from the lynx spider Oxyopes takobius: Structure of spiderine domains in solution and membrane-mimicking environment.,Nadezhdin KD, Romanovskaia DD, Sachkova MY, Oparin PB, Kovalchuk SI, Grishin EV, Arseniev AS, Vassilevski AA Protein Sci. 2017 Mar;26(3):611-616. doi: 10.1002/pro.3101. Epub 2017 Feb 12. PMID:27997708<ref>PMID:27997708</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

== References ==

__TOC__

</StructureSection>

[[Category: Large Structures]]

[[Category: Arseniev, A]]

[[Category: Grishin, E]]

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 ==NMR structure of OtTx1a - AMP in DPC micelles==
-<StructureSection load='2n85' size='340' side='right' caption='[[2n85]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2n85' size='340' side='right'caption='[[2n85]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2n85]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N85 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N85 FirstGlance]. <br>
@@ Line 7: / Line 7: @@
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n85 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n85 OCA], [http://pdbe.org/2n85 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n85 RCSB], [http://www.ebi.ac.uk/pdbsum/2n85 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2n85 ProSAT]</span></td></tr>
 </table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+We have recently demonstrated that a common phenomenon in evolution of spider venom composition is the emergence of so-called modular toxins consisting of two domains, each corresponding to a "usual" single-domain toxin. In this article, we describe the structure of two domains that build up a modular toxin named spiderine or OtTx1a from the venom of Oxyopes takobius. Both domains were investigated by solution NMR in water and detergent micelles used to mimic membrane environment. The N-terminal spiderine domain OtTx1a-AMP (41 amino acid residues) contains no cysteines. It is disordered in aqueous solution but in micelles, it assumes a stable amphiphilic structure consisting of two alpha-helices separated by a flexible linker. On the contrary, the C-terminal domain OtTx1a-ICK (59 residues) is a disulfide-rich polypeptide reticulated by five S-S bridges. It presents a stable structure in water and its core is the inhibitor cystine knot (ICK) or knottin motif that is common among single-domain neurotoxins. OtTx1a-ICK structure is the first knottin with five disulfide bridges and it represents a good reference for the whole oxytoxin family. The affinity of both domains to membranes was measured with NMR using titration by liposome suspensions. In agreement with biological tests, OtTx1a-AMP was found to show high membrane affinity explaining its potent antimicrobial properties.
+Modular toxin from the lynx spider Oxyopes takobius: Structure of spiderine domains in solution and membrane-mimicking environment.,Nadezhdin KD, Romanovskaia DD, Sachkova MY, Oparin PB, Kovalchuk SI, Grishin EV, Arseniev AS, Vassilevski AA Protein Sci. 2017 Mar;26(3):611-616. doi: 10.1002/pro.3101. Epub 2017 Feb 12. PMID:27997708<ref>PMID:27997708</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2n85" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Arseniev, A]]
 [[Category: Grishin, E]]