User:Sean Callahan/Sandbox 1: Difference between revisions
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==Structure== | ==Structure== | ||
The LSD1 crystal structure was obtained in the presence of its FAD cofactor. To ensure proper formation, crystals were created in the presence of Hg ions. LSD1 contains both conserved and novel structural features. Its substrates are mono- or di-methylated lysine residues and its products are unmethylated or monomethylated lysines, respectively. | |||
===N-Terminus=== | ===N-Terminus=== | ||
Going in order of primary structure, the first ~166 residues are believed to be unstructured and contain a nuclear localization signal. This area of the protein has also been shown to be susceptible to proteolytic cleavage, which may be to remove the localization signal and render protein inactive. However, a mutant of LSD1, which contains residues 166-852 (essentially eliminating the unstructured region) has been shown to be stable and viable when compared to wild-type LSD1 in a photometric activity assay. Unfortunately, this portion of the protein was unable to be crystallized. | Going in order of primary structure, the first ~166 residues are believed to be unstructured and contain a nuclear localization signal. This area of the protein has also been shown to be susceptible to proteolytic cleavage, which may be to remove the localization signal and render protein inactive. However, a mutant of LSD1, which contains residues 166-852 (essentially eliminating the unstructured region) has been shown to be stable and viable when compared to wild-type LSD1 in a photometric activity assay. Unfortunately, this portion of the protein was unable to be crystallized. | ||
