4qe7

Open MthK pore structure soaked in 10 mM Ba2+/100 mM Na+

spinqualitylabelsall models 4qe7, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4qe7 is a 1 chain structure with sequence from Metth. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: BA Related: 3ldc, 3ldd, 3lde, 4qe9 Gene: mthK, MTH_1520 (METTH) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [MTHK_METTH] Calcium-gated potassium channel. Publication Abstract from PubMed The movement and interaction of multiple ions passing through in single file underlie various fundamental K(+) channel properties, from the effective conduction of K(+) ions to channel blockade by Ba(2+) ions. In this study, we used single-channel electrophysiology and x-ray crystallography to probe the interactions of Ba(2+) with permeant ions within the ion conduction pathway of the MthK K(+) channel. We found that, as typical of K(+) channels, the MthK channel was blocked by Ba(2+) at the internal side, and the Ba(2+)-blocking effect was enhanced by external K(+). We also obtained crystal structures of the MthK K(+) channel pore in both Ba(2+)-Na(+) and Ba(2+)-K(+) environments. In the Ba(2+)-Na(+) environment, we found that a single Ba(2+) ion remained bound in the selectivity filter, preferably at site 2, whereas in the Ba(2+)-K(+) environment, Ba(2+) ions were predominantly distributed between sites 3 and 4. These ionic configurations are remarkably consistent with the functional studies and identify a molecular basis for Ba(2+) blockade of K(+) channels. Ionic interactions of Ba2+ blockades in the MthK K+ channel.,Guo R, Zeng W, Cui H, Chen L, Ye S J Gen Physiol. 2014 Aug;144(2):193-200. doi: 10.1085/jgp.201411192. Epub 2014 Jul, 14. PMID:25024268 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Potassium Channel

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