User:Madeleine Wilson/Sandbox 1: Difference between revisions
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===The Active Site=== | ===The Active Site=== | ||
The active site and binding pocket of KMT | The active site and binding pocket of KMT contain residues and shape that ensure both catalytic capability as well as optimal stability. First, the lysine of the histone enters the active site via the <scene name='81/811092/Tyrosine_channel_2/3'>Lysine access channel</scene> comprised of Tyr335 and Tyr337. Once in the active site, the alkyl part of the histone chain is stabilized by the <scene name='81/811092/Hydrophobic_binding_pocket/1'>hydrophobic binding pocket</scene>, and polar residues are stabilized by hydrogen bonding interactions on the surface. The Tyr335 and Tyr337 are also essential for stabilization of histone chain via hydrogen bonding. | ||
The <scene name='81/811092/Active_site_w_water/2'>active site</scene> itself contains the cofactor [https://en.wikipedia.org/wiki/S-Adenosyl_methionine S-adenosyl methionine (SAM)] which donates the methyl group in the reaction. <ref name="Xiao" /> | The <scene name='81/811092/Active_site_w_water/2'>active site</scene> itself contains the cofactor [https://en.wikipedia.org/wiki/S-Adenosyl_methionine S-adenosyl methionine (SAM)] which donates the methyl group in the reaction. <ref name="Xiao" /> | ||
[[Image:KMT_mechanism.png|200px|left|thumb|KMT Mechanism]] | [[Image:KMT_mechanism.png|200px|left|thumb|KMT Mechanism]] | ||
