User:Madeleine Wilson/Sandbox 1: Difference between revisions
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The <scene name='81/811092/Active_site_w_water/3'>active site</scene> itself contains the cofactor [https://en.wikipedia.org/wiki/S-Adenosyl_methionine S-adenosyl methionine (SAM)] which donates the methyl group in the reaction. <ref name="Xiao" /> In the active site scene, the strict depicts the post-reaction result, where the Lys has been methylated and SAM has been converted to S-adenosyl homocysteine (SAH). | The <scene name='81/811092/Active_site_w_water/3'>active site</scene> itself contains the cofactor [https://en.wikipedia.org/wiki/S-Adenosyl_methionine S-adenosyl methionine (SAM)] which donates the methyl group in the reaction. <ref name="Xiao" /> In the active site scene, the strict depicts the post-reaction result, where the Lys has been methylated and SAM has been converted to S-adenosyl homocysteine (SAH). | ||
[[Image:KMT_Mechanism_jpg.jpeg|200px|left|thumb|Figure 2. KMT Mechanism]] | [[Image:KMT_Mechanism_jpg.jpeg|200px|left|thumb|Figure 2. KMT Mechanism]] | ||
The reaction is catalyzed by Tyr305, Tyr245, carbonyl oxygens of the main chain in residues | The reaction is catalyzed by Tyr305, Tyr245, carbonyl oxygens of the main chain in residues Ala295 and Ser290. Tyr305 and the carbonyl oxygens stabilize and pull electron density off a water to pull on one of the hydrogens off the nitrogen of the lysine, while oxygen of Tyr245 pulls on the other hydrogen of the nitrogen. Both of these actions allow nitrogen to become more nucleophilic and attack the carbon of the methyl group on the SAM, which is attached to a positively charged sulfur. The methyl group is then transferred and the sulfur is neutral; SAM has been converted to (SAH). <ref name="Xiao" /> | ||
==Inhibitors== | ==Inhibitors== | ||
