6j46: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 6j46 is ~~ON HOLD until Paper Publication~~

==LepI-SAH complex structure==

~~Authors~~: ~~Qiu~~, S., ~~Wei~~, C.

== Structural highlights ==

<table><tr><td colspan='2'>[[6j46]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J46 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6J46 FirstGlance]. <br>

~~Description~~: LepI-~~SAH complex structure~~

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>

[[Category: ~~Unreleased~~ Structures]]

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6j46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j46 OCA], [http://pdbe.org/6j46 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j46 RCSB], [http://www.ebi.ac.uk/pdbsum/6j46 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j46 ProSAT]</span></td></tr>

</table>

== Function ==

[[http://www.uniprot.org/uniprot/LEPI_ASPFN LEPI_ASPFN]] O-methyltransferase; part of the gene cluster 23 that mediates the biosynthesis of a family of 2-pyridones known as leporins (PubMed:20447271, PubMed:26051490). The hybrid PKS-NRPS synthetase lepA and the enoyl reductase lepG are responsible for fusion of phenylalanine with a hexaketide and subsequent release of the stable tetramic acid precursor, pre-leporin C (PubMed:26051490). Because lepA lacks a designated enoylreductase (ER) domain, the required activity is provided the enoyl reductase lepG (PubMed:26051490). It is possible that the dehydrogenase lepF also participates in production of pre-leporin C (PubMed:26051490). Cytochrome P450 monooxygenase lepH is then required for the ring expansion step to yield leporin C (PubMed:26051490). Leporin C is then presumably further oxidized by the N-hydroxylase lepD to form leporin B (PubMed:26051490). LepI may possess a function in biosynthesis upstream of lepA (PubMed:26051490). Leporin B is further oxidized in the presence of ferric ion to give the leporin B trimer-iron chelate, but whether or not this reaction is catalyzed by an enzyme in the pathway or by ferric ion is not determined yet (PubMed:26051490).<ref>PMID:26051490</ref> <ref>PMID:20447271</ref>

== References ==

__TOC__

</StructureSection>

[[Category: Large Structures]]

[[Category: Qiu, S]]

[[Category: Wei, C]]

[[Category: Complex]]

[[Category: Transferase]]

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 6j46 is ON HOLD  until Paper Publication
+==LepI-SAH complex structure==
+<StructureSection load='6j46' size='340' side='right'caption='[[6j46]], [[Resolution|resolution]] 2.62&Aring;' scene=''>
-Authors: Qiu, S., Wei, C.
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6j46]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J46 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6J46 FirstGlance]. <br>
-Description: LepI-SAH complex structure
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
-[[Category: Unreleased Structures]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6j46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j46 OCA], [http://pdbe.org/6j46 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j46 RCSB], [http://www.ebi.ac.uk/pdbsum/6j46 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j46 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/LEPI_ASPFN LEPI_ASPFN]] O-methyltransferase; part of the gene cluster 23 that mediates the biosynthesis of a family of 2-pyridones known as leporins (PubMed:20447271, PubMed:26051490). The hybrid PKS-NRPS synthetase lepA and the enoyl reductase lepG are responsible for fusion of phenylalanine with a hexaketide and subsequent release of the stable tetramic acid precursor, pre-leporin C (PubMed:26051490). Because lepA lacks a designated enoylreductase (ER) domain, the required activity is provided the enoyl reductase lepG (PubMed:26051490). It is possible that the dehydrogenase lepF also participates in production of pre-leporin C (PubMed:26051490). Cytochrome P450 monooxygenase lepH is then required for the ring expansion step to yield leporin C (PubMed:26051490). Leporin C is then presumably further oxidized by the N-hydroxylase lepD to form leporin B (PubMed:26051490). LepI may possess a function in biosynthesis upstream of lepA (PubMed:26051490). Leporin B is further oxidized in the presence of ferric ion to give the leporin B trimer-iron chelate, but whether or not this reaction is catalyzed by an enzyme in the pathway or by ferric ion is not determined yet (PubMed:26051490).<ref>PMID:26051490</ref> <ref>PMID:20447271</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Qiu, S]]
 [[Category: Wei, C]]
+[[Category: Complex]]
+[[Category: Transferase]]