Sandbox Reserved 1546: Difference between revisions
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='''5H86: HUMAN GCN-5 BOUND TO BUTYRYL-COA'''= | ='''5H86: HUMAN GCN-5 BOUND TO BUTYRYL-COA'''= | ||
This project was completed by Marianne Javier, Karima MuhummadPoe, and Makayla Yang for Dr. Gluick's Spring 2019 BIOL4100K-01 class at Georgia Gwinnett College. | This project was completed by Marianne Javier, Karima MuhummadPoe, and Makayla Yang for Dr. Gluick's Spring 2019 BIOL4100K-01 class at Georgia Gwinnett College. | ||
<p>'''Project Purpose:''' We chose this molecule due to its role as a histone acetyltransferase and its role in transcription regulation <ref>doi: 10.1107/S2059798316007907</ref>. It has a harder time having successful enzymatic activity if the acyl-chain is too long which is the purpose of studying the molecule <ref name = " Structural basis for acyl-group discrimination by human Gcn5L2/>. These researchers made two different acyl-CoA molecules, propionyl-CoA and butyryl-CoA <ref | <p>'''Project Purpose:''' We chose this molecule due to its role as a histone acetyltransferase and its role in transcription regulation <ref name = "Structural basis for acyl-group discrimination by human Gcn5L2">doi: 10.1107/S2059798316007907</ref>. It has a harder time having successful enzymatic activity if the acyl-chain is too long which is the purpose of studying the molecule <ref name = " Structural basis for acyl-group discrimination by human Gcn5L2/>. These researchers made two different acyl-CoA molecules, propionyl-CoA and butyryl-CoA <ref name = " Structural basis for acyl-group discrimination by human Gcn5L2/>. We are looking at the butyryl-CoA which has a conformation that obstructs the lysine from binding. We chose this molecule to see how Gcn5L2 chooses which acyl-chain donor to have the highest enzymatic activity.</p> | ||
==Function== | ==Function== | ||
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<p> 1. '''Lipolysis and release from adipose tissue''': In the initial steps of degradation, fatty acids are stored in adipocytes. The breakdown of adipocytes is called lipolysis (They are released into blood stream to circulate through body.)</p> | <p> 1. '''Lipolysis and release from adipose tissue''': In the initial steps of degradation, fatty acids are stored in adipocytes. The breakdown of adipocytes is called lipolysis (They are released into blood stream to circulate through body.)</p> | ||
<p> 2. '''Activation and transport into the mitochondria''': The mitochondria is where fatty acid oxidation occurs which activates fatty acids to be carried to the mitochondria. The enzyme responsible for the catalysis of this step is Fatty-Acyl Coa Synthetase. Malonyl ACP is the activated donor of two carbon units in the elongation step which is operated by the release of CO2.</p> | <p> 2. '''Activation and transport into the mitochondria''': The mitochondria is where fatty acid oxidation occurs which activates fatty acids to be carried to the mitochondria. The enzyme responsible for the catalysis of this step is Fatty-Acyl Coa Synthetase. Malonyl ACP is the activated donor of two carbon units in the elongation step which is operated by the release of CO2.</p> | ||
<p>3. '''Β-oxidation''': Once inside mitochodria, five steps occur: 1) Activation by ATP, 2) Oxydation by FAD, 3) Hydration, 4) Oxydation by NAD+, and 5) Thyolysis. The final product is Acetyl-Coa which is now able to be able to enter the TCA cycle <ref>Berg JM, Tymoczko JL, Stryer L. Biochemistry. 5th edition. New York: W H Freeman; 2002. Section 22.4, Fatty Acids Are Synthesized and Degraded by Different Pathways. Available from: https://www.ncbi.nlm.nih.gov/books/NBK22554/</ref>. </p> | <p>3. '''Β-oxidation''': Once inside mitochodria, five steps occur: 1) Activation by ATP, 2) Oxydation by FAD, 3) Hydration, 4) Oxydation by NAD+, and 5) Thyolysis. The final product is Acetyl-Coa which is now able to be able to enter the TCA cycle <ref name = "Section 22.4 Fatty Acids Are Synthesized and Degraded by Different Pathways">Berg JM, Tymoczko JL, Stryer L. Biochemistry. 5th edition. New York: W H Freeman; 2002. Section 22.4, Fatty Acids Are Synthesized and Degraded by Different Pathways. Available from: https://www.ncbi.nlm.nih.gov/books/NBK22554/</ref>. </p> | ||
