Sandbox Reserved 1546: Difference between revisions

<p>3. '''Β-oxidation''': Once inside the mitochodria, five steps occur: 1) Activation by ATP, 2) Oxidation by FAD, 3) Hydration, 4) Oxidation by NAD+, and 5) Thyolysis. The final product is Acetyl-CoA which is now able to be able to enter the TCA cycle <ref name = "Section 22.4 Fatty Acids Are Synthesized and Degraded by Different Pathways">Berg JM, Tymoczko JL, Stryer L. Biochemistry. 5th edition. New York: W H Freeman; 2002. Section 22.4, Fatty Acids Are Synthesized and Degraded by Different Pathways. Available from: https://www.ncbi.nlm.nih.gov/books/NBK22554/</ref>. </p>

<p>One of the enzymes that have a key role in fatty acid degradation and that is also our enzyme of study is known as acyl-CoA acetyltransferase or thiolase. Thiolases are enzymes that have key roles in biochemical pathways and can either be degrative or biosynthetic. Degrative thiolases such as 3-ketoacyl-CoA thiolase, plays a role in fatty-acid β-oxidation in peroxisomes and mitochondria and ketone body metabolism in mitochondria, whereas biosynthetic thiolases, like acetoacetyl-CoA thiolase, catalyzes Acetoacetyl-CoA to two Acetyl-CoAs.</p>