2bf8: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2bf8.gif|left|200px]] | [[Image:2bf8.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_2bf8", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
or leave the SCENE parameter empty for the default display. | |||
| | --> | ||
| | {{STRUCTURE_2bf8| PDB=2bf8 | SCENE= }} | ||
}} | |||
'''CRYSTAL STRUCTURE OF SUMO MODIFIED UBIQUITIN CONJUGATING ENZYME E2-25K''' | '''CRYSTAL STRUCTURE OF SUMO MODIFIED UBIQUITIN CONJUGATING ENZYME E2-25K''' | ||
| Line 37: | Line 34: | ||
[[Category: SPINE, Structural Proteomics in Europe.]] | [[Category: SPINE, Structural Proteomics in Europe.]] | ||
[[Category: Sixma, T K.]] | [[Category: Sixma, T K.]] | ||
[[Category: | [[Category: E2 ubiquitin conjugating enzyme]] | ||
[[Category: | [[Category: E2-25k]] | ||
[[Category: | [[Category: Ligase]] | ||
[[Category: | [[Category: Spine]] | ||
[[Category: | [[Category: Structural genomic]] | ||
[[Category: | [[Category: Structural proteomics in europe]] | ||
[[Category: | [[Category: Sumo]] | ||
[[Category: | [[Category: Sumo-target structure]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Apr 13 08:17:54 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 05:17, 13 April 2008
CRYSTAL STRUCTURE OF SUMO MODIFIED UBIQUITIN CONJUGATING ENZYME E2-25K
Overview
Post-translational modification with small ubiquitin-related modifier (SUMO) alters the function of many proteins, but the molecular mechanisms and consequences of this modification are still poorly defined. During a screen for novel SUMO1 targets, we identified the ubiquitin-conjugating enzyme E2-25K (Hip2). SUMO attachment severely impairs E2-25K ubiquitin thioester and unanchored ubiquitin chain formation in vitro. Crystal structures of E2-25K(1-155) and of the E2-25K(1-155)-SUMO conjugate (E2-25K(*)SUMO) indicate that SUMO attachment interferes with E1 interaction through its location on the N-terminal helix. The SUMO acceptor site in E2-25K, Lys14, does not conform to the consensus site found in most SUMO targets (PsiKXE), and functions only in the context of an alpha-helix. In contrast, adjacent SUMO consensus sites are modified only when in unstructured peptides. The demonstration that secondary structure elements are part of SUMO attachment signals could contribute to a better prediction of SUMO targets.
About this Structure
2BF8 is a Protein complex structure of sequences from Bos taurus and Homo sapiens. Full crystallographic information is available from OCA.
Reference
SUMO modification of the ubiquitin-conjugating enzyme E2-25K., Pichler A, Knipscheer P, Oberhofer E, van Dijk WJ, Korner R, Olsen JV, Jentsch S, Melchior F, Sixma TK, Nat Struct Mol Biol. 2005 Mar;12(3):264-9. Epub 2005 Feb 20. PMID:15723079 Page seeded by OCA on Sun Apr 13 08:17:54 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Bos taurus
- Homo sapiens
- Protein complex
- Ubiquitin--protein ligase
- Dijk, W J.Van.
- Jentsch, S.
- Knipscheer, P.
- Korner, R.
- Melchior, F.
- Oberhofer, E.
- Olsen, J Velgaard.
- Pichler, A.
- SPINE, Structural Proteomics in Europe.
- Sixma, T K.
- E2 ubiquitin conjugating enzyme
- E2-25k
- Ligase
- Spine
- Structural genomic
- Structural proteomics in europe
- Sumo
- Sumo-target structure
