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'''STRUCTURE AND FUNCTION OF RV0130, A CONSERVED HYPOTHETICAL PROTEIN FROM M.TUBERCULOSIS''' | '''STRUCTURE AND FUNCTION OF RV0130, A CONSERVED HYPOTHETICAL PROTEIN FROM M.TUBERCULOSIS''' | ||
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[[Category: Jones, T A.]] | [[Category: Jones, T A.]] | ||
[[Category: SPINE, Structural Proteomics in Europe.]] | [[Category: SPINE, Structural Proteomics in Europe.]] | ||
[[Category: | [[Category: Conserved hypothetical protein]] | ||
[[Category: | [[Category: Hotdog]] | ||
[[Category: | [[Category: Hydratase]] | ||
[[Category: | [[Category: Lyase]] | ||
[[Category: | [[Category: Rv0130]] | ||
[[Category: | [[Category: Spine]] | ||
[[Category: | [[Category: Structural genomic]] | ||
[[Category: | [[Category: Structural proteomics in europe]] | ||
[[Category: | [[Category: Tuberculosis]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Apr 13 08:18:47 2008'' | |||
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Revision as of 05:18, 13 April 2008
STRUCTURE AND FUNCTION OF RV0130, A CONSERVED HYPOTHETICAL PROTEIN FROM M.TUBERCULOSIS
Overview
A large fraction of the Mycobacterium tuberculosis genome codes for proteins of unknown function. We here report the structure of one of these proteins, Rv0130, solved to a resolution of 1.8 a. The Rv0130 monomer features a single hotdog fold composed of a highly curved beta-sheet on top of a long and a short alpha-helix. Two monomers in turn pack to form a double-hotdog-folded homodimer, similar to a large group of enzymes that use thiol esters as substrates. Rv0130 was found to contain a highly conserved R-specific hydratase motif buried deeply between the two monomers. Our biochemical studies show that the protein is able to hydrate a short trans-2-enoyl-coenzyme A moiety with a k(cat) of 1.1 x 10(2) sec(-1). The importance of the side chains of D40 and H45 for hydratase activity is demonstrated by site-directed mutagenesis. In contrast to many hotdog-folded proteins, a proline residue distorts the central helix of Rv0130. This distortion allows the creation of a long, curved tunnel, similar to the substrate-binding channels of long-chain eukaryotic hydratase 2 enzymes.
About this Structure
2C2I is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.
Reference
Structure and function of Rv0130, a conserved hypothetical protein from Mycobacterium tuberculosis., Johansson P, Castell A, Jones TA, Backbro K, Protein Sci. 2006 Oct;15(10):2300-9. Epub 2006 Sep 8. PMID:16963641 Page seeded by OCA on Sun Apr 13 08:18:47 2008
