Resolution: Difference between revisions

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~~Resolution~~ is the smallest distance between crystal lattice planes that is resolved in the diffraction pattern. High numeric values of resolution, such as 4 Å, mean poor resolution, while low numeric values, such as 1.5 Å, mean good resolution.

Structure determination by [[X-ray crystallography]] or [[cryo-electron microscopy]] produces an [[electron density map]] (shown above in <font color="#508050"><b>green</b></font>). The atomic model, shown as sticks above, is then built, guided by the electron density map.

In X-ray crystallography, resolution is the smallest distance between crystal lattice planes that is resolved in the diffraction pattern. High numeric values of resolution, such as 4 Å, mean poor resolution, while low numeric values, such as 1.5 Å, mean good resolution.

'''2.05 Å''' is the '''median''' resolution for [[X-ray crystallography|X-ray crystallographic]] results in the Protein Data Bank (135,762 on May 19, 2019). For comparison, the van der Waals diameter of a carbon atom is 3.4-3.7 Å<ref name="atomsize">See [http://en.wikipedia.org/wiki/Atomic_radii_of_the_elements_(data_page) Atomic radii of the elements (Wikipedia)], and [http://chemwiki.ucdavis.edu/Theoretical_Chemistry/Chemical_Bonding/General_Principles/Covalent_Bond_Distance,_Radius_and_van_der_Waals_Radius values from an Inorganic Chemistry textbook].</ref>, and the length of a covalent carbon-carbon bond is 1.5 Å<ref name="atomsize" />.

Resolution: Difference between revisions

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-Resolution is the smallest distance between crystal lattice planes that is resolved in the diffraction pattern. High numeric values of resolution, such as 4 Å, mean poor resolution, while low numeric values, such as 1.5 Å, mean good resolution.
+Structure determination by [[X-ray crystallography]] or [[cryo-electron microscopy]] produces an [[electron density map]] (shown above in <font color="#508050"><b>green</b></font>). The atomic model, shown as sticks above, is then built, guided by the electron density map.
+In X-ray crystallography, resolution is the smallest distance between crystal lattice planes that is resolved in the diffraction pattern. High numeric values of resolution, such as 4 Å, mean poor resolution, while low numeric values, such as 1.5 Å, mean good resolution.
 '''2.05 Å''' is the '''median''' resolution for [[X-ray crystallography|X-ray crystallographic]] results in the Protein Data Bank (135,762 on May 19, 2019). For comparison, the van der Waals diameter of a carbon atom is 3.4-3.7 &Aring;<ref name="atomsize">See [http://en.wikipedia.org/wiki/Atomic_radii_of_the_elements_(data_page) Atomic radii of the elements (Wikipedia)], and [http://chemwiki.ucdavis.edu/Theoretical_Chemistry/Chemical_Bonding/General_Principles/Covalent_Bond_Distance,_Radius_and_van_der_Waals_Radius values from an Inorganic Chemistry textbook].</ref>, and the length of a covalent carbon-carbon bond is 1.5 &Aring;<ref name="atomsize" />.