6niv: Difference between revisions
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==Racemic Phenol-Soluble Modulin Alpha 3 Peptide== | |||
<StructureSection load='6niv' size='340' side='right'caption='[[6niv]], [[Resolution|resolution]] 1.45Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6niv]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NIV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NIV FirstGlance]. <br> | |||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6niv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6niv OCA], [http://pdbe.org/6niv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6niv RCSB], [http://www.ebi.ac.uk/pdbsum/6niv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6niv ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Phenol-soluble modulin alpha3 (PSMalpha3) is a cytotoxic peptide secreted by virulent strains of Staphylococcus aureus. We used a stereochemical strategy to examine the mechanism of PSMalpha3-mediated toxicity. One hypothesis is that PSMalpha3 toxicity requires fibril formation; an alternative is that toxicity is caused by soluble forms of PSMalpha3, possibly oligomeric. We find that the unnatural enantiomer (D residues) displays cytotoxicity comparable to that of L-PSMalpha3. Racemic PSMalpha3 is similarly toxic to enantiopure PSMalpha3 (L or D) under some conditions, but the toxicity is lost under conditions that cause racemic PSMalpha3 to aggregate. A crystal structure of racemic PSMalpha3-NH2 displays an alpha-helical secondary structure and a packing pattern that is reminiscent of the cross-alpha arrangement recently discovered in crystals of L-PSMalpha3. Our data suggest that the cytotoxicity of PSMalpha3 does not depend on stereospecific engagement of a target protein or other chiral macromolecule, an observation that supports a mechanism based on membrane disruption. In addition, our data support the hypothesis that toxicity is exerted by a soluble form rather than an insoluble fibrillar form. | |||
Use of a Stereochemical Strategy To Probe the Mechanism of Phenol-Soluble Modulin alpha3 Toxicity.,Yao Z, Cary BP, Bingman CA, Wang C, Kreitler DF, Satyshur KA, Forest KT, Gellman SH J Am Chem Soc. 2019 May 15;141(19):7660-7664. doi: 10.1021/jacs.9b00349. Epub, 2019 May 2. PMID:31045358<ref>PMID:31045358</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6niv" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Bingman, C A]] | |||
[[Category: Cary, B P]] | |||
[[Category: Forest, K T]] | |||
[[Category: Gellman, S H]] | |||
[[Category: Kreitler, D F]] | |||
[[Category: Satyshur, K A]] | |||
[[Category: Wang, C]] | [[Category: Wang, C]] | ||
[[Category: Yao, Z]] | [[Category: Yao, Z]] | ||
[[Category: | [[Category: Peptide toxin]] | ||
[[Category: | [[Category: Protein fibril]] | ||
[[Category: | [[Category: Racemic crystallization]] | ||
Revision as of 07:33, 21 May 2019
Racemic Phenol-Soluble Modulin Alpha 3 Peptide
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