6rim: Difference between revisions
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==Crystal structure of the catalytic domain of the Weissela oryzae botulinum like toxin== | |||
<StructureSection load='6rim' size='340' side='right'caption='[[6rim]], [[Resolution|resolution]] 1.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6rim]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RIM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RIM FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rim OCA], [http://pdbe.org/6rim PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rim RCSB], [http://www.ebi.ac.uk/pdbsum/6rim PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rim ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/BXWO_WEIOS BXWO_WEIOS]] When overexpressed the N-terminus (residues 1-476) cleaves rat synaptobrevin-2/VAMP2 between '89-Trp-|-Trp-90' in vitro. This releases the cytoplasmic domain of VAMP2 from the synaptic vesicle membrane, which would prevent the assembly of the trans-SNARE complex on the membrane and thus prevent vesicle-target membrane fusion and neurotransmitter release (PubMed:27443638).<ref>PMID:27443638</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Botulinum neurotoxins (BoNTs) are the most potent toxins known. So far, eight serotypes have been identified that all act as zinc-dependent endopeptidases targeting SNARE proteins and inhibiting the release of neurotransmitters. Recently, the first botulinum toxin-like protein was identified outside the Clostridial genus, designated BoNT/Wo in the genome of Weissella oryzae. Here, we report the 1.6 A X-ray crystal structure of the light chain of BoNT/Wo (LC/Wo). LC/Wo presents the core fold common to BoNTs but has an unusually wide, open, and negatively charged catalytic pocket, with an additional Ca(2+) ion besides the zinc ion and a unique ss-hairpin motif. The structural information will help establish the substrate profile of BoNT/Wo and help our understanding of how BoNT evolved. This article is protected by copyright. All rights reserved. | |||
Crystal structure of the catalytic domain of the Weissela oryzae botulinum-like toxin.,Kosenina S, Masuyer G, Zhang S, Dong M, Stenmark P FEBS Lett. 2019 May 20. doi: 10.1002/1873-3468.13446. PMID:31111466<ref>PMID:31111466</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6rim" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bontoxilysin]] | |||
[[Category: Large Structures]] | |||
[[Category: Kosenina, S]] | |||
[[Category: Masuyer, G]] | |||
[[Category: Stenmark, P]] | |||
[[Category: Botulinum neurotoxin]] | |||
[[Category: Toxin]] | |||
[[Category: Zinc endopeptidase]] | |||
Revision as of 06:23, 29 May 2019
Crystal structure of the catalytic domain of the Weissela oryzae botulinum like toxin
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