6rjh: Difference between revisions
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==3D structure of horse spleen apoferritin determined using multifunctional graphene supports for electron cryomicroscopy== | |||
<StructureSection load='6rjh' size='340' side='right'caption='[[6rjh]], [[Resolution|resolution]] 2.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6rjh]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RJH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RJH FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rjh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rjh OCA], [http://pdbe.org/6rjh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rjh RCSB], [http://www.ebi.ac.uk/pdbsum/6rjh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rjh ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/FRIL_HORSE FRIL_HORSE]] Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity). | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
With recent technological advances, the atomic resolution structure of any purified biomolecular complex can, in principle, be determined by single-particle electron cryomicroscopy (cryoEM). In practice, the primary barrier to structure determination is the preparation of a frozen specimen suitable for high-resolution imaging. To address this, we present a multifunctional specimen support for cryoEM, comprising large-crystal monolayer graphene suspended across the surface of an ultrastable gold specimen support. Using a low-energy plasma surface modification system, we tune the surface of this support to the specimen by patterning a range of covalent functionalizations across the graphene layer on a single grid. This support design reduces specimen movement during imaging, improves image quality, and allows high-resolution structure determination with a minimum of material and data. | |||
Multifunctional graphene supports for electron cryomicroscopy.,Naydenova K, Peet MJ, Russo CJ Proc Natl Acad Sci U S A. 2019 May 24. pii: 1904766116. doi:, 10.1073/pnas.1904766116. PMID:31127045<ref>PMID:31127045</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6rjh" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Equus caballus]] | |||
[[Category: Large Structures]] | |||
[[Category: Naydenova, K]] | |||
[[Category: Peet, M J]] | |||
[[Category: Russo, C J]] | |||
[[Category: Metal transport]] | |||
