6o30: Difference between revisions
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==Lipid A transporter MsbA from Salmonella typhimurium== | |||
<StructureSection load='6o30' size='340' side='right'caption='[[6o30]], [[Resolution|resolution]] 4.47Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6o30]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O30 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6O30 FirstGlance]. <br> | |||
</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/ABC-type_lipid_A-core_oligosaccharide_transporter ABC-type lipid A-core oligosaccharide transporter], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=7.5.2.6 7.5.2.6] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6o30 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o30 OCA], [http://pdbe.org/6o30 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o30 RCSB], [http://www.ebi.ac.uk/pdbsum/6o30 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o30 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/A0A0K6RU95_SALTM A0A0K6RU95_SALTM]] Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.[HAMAP-Rule:MF_01703][SAAS:SAAS00055332] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
MsbA is an essential ATP-binding cassette transporter in Gram-negative bacteria that transports lipid A and lipopolysaccharide from the cytoplasmic leaflet to the periplasmic leaflet of the inner membrane. Here we report the X-ray structure of MsbA from Salmonella typhimurium at 2.8-A resolution in an inward-facing conformation after cocrystallization with lipid A and using a stabilizing facial amphiphile. The structure displays a large amplitude opening in the transmembrane portal, which is likely required for lipid A to pass from its site of synthesis into the protein-enclosed transport pathway. Putative lipid A density is observed further inside the transmembrane cavity, consistent with a trap and flip model. Additional electron density attributed to lipid A is observed near an outer surface cleft at the periplasmic ends of the transmembrane helices. These findings provide new structural insights into the lipid A transport pathway through comparative analysis with existing MsbA structures. | |||
Structural Insights into the Lipid A Transport Pathway in MsbA.,Padayatti PS, Lee SC, Stanfield RL, Wen PC, Tajkhorshid E, Wilson IA, Zhang Q Structure. 2019 Apr 25. pii: S0969-2126(19)30129-7. doi:, 10.1016/j.str.2019.04.007. PMID:31130486<ref>PMID:31130486</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6o30" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: ABC-type lipid A-core oligosaccharide transporter]] | |||
[[Category: Large Structures]] | |||
[[Category: Lee, S C]] | |||
[[Category: Padayatti, P S]] | |||
[[Category: Stanfield, R L]] | |||
[[Category: Wilson, I A]] | |||
[[Category: Zhang, Q]] | |||
[[Category: Abc transporter]] | |||
[[Category: Lipid a co-crystallization]] | |||
[[Category: Membrane protein]] | |||
[[Category: Msba]] | |||
[[Category: Staphylococcus typhimurium]] | |||
Revision as of 05:42, 12 June 2019
Lipid A transporter MsbA from Salmonella typhimurium
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