5nhk

Structure of Ferric uptake regulator from francisella tularensis with Iron

spinqualitylabelsall models 5nhk, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5nhk is a 4 chain structure with sequence from "bacterium_tularense"_mccoy_and_chapin_1912 "bacterium tularense" mccoy and chapin 1912. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: FE, ZN Gene: fur, AV531_00680, DR86_1441 ("Bacterium tularense" McCoy and Chapin 1912) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Francisella tularensis is a Gram-negative bacterium causing tularaemia. Classified as possible bioterrorism agent, it may be transmitted to humans via animal infection or inhalation leading to severe pneumonia. Its virulence is related to iron homeostasis involving siderophore biosynthesis directly controlled at the transcription level by the ferric uptake regulator Fur, as presented here together with the first crystal structure of the tetrameric F. tularensis Fur in the presence of its physiological cofactor, Fe(2+). Through structural, biophysical, biochemical and modelling studies, we show that promoter sequences of F. tularensis containing Fur boxes enable this tetrameric protein to bind them by splitting it into two dimers. Furthermore, the critical role of F. tularensis Fur in virulence and pathogenesis is demonstrated with a fur-deleted mutant showing an attenuated virulence in macrophage-like cells and mice. Together, our study suggests that Fur is an attractive target of new antibiotics that attenuate the virulence of F. tularensis. Structural and functional studies of the metalloregulator Fur identify a promoter-binding mechanism and its role in Francisella tularensis virulence.,Perard J, Nader S, Levert M, Arnaud L, Carpentier P, Siebert C, Blanquet F, Cavazza C, Renesto P, Schneider D, Maurin M, Coves J, Crouzy S, Michaud-Soret I Commun Biol. 2018 Jul 17;1:93. doi: 10.1038/s42003-018-0095-6. eCollection 2018. PMID:30271974 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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