Parkin: Difference between revisions

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==Parkin==
==Parkin==
<StructureSection load='4i1h' size='340' side='right' caption='Parkin (PDB code: 5c1z)' scene=''>
<StructureSection load='4i1h' size='340' side='right' caption='Parkin (PDB code: 5c1z)' scene='"/12/3456/Sample/1"'>
Domains: Ubl, RING0, RING1, IBR, REP, RING2.
Parkin is a protein of the RBR protein family.
Parkin is a protein of the RBR protein family.


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== Structural highlights ==
== Structural highlights ==


Clicking <scene name="/12/3456/Sample/1">here</scene> will colour Parkin from N-terminal (bluer) to C-terminal (redder) residues. <scene name='81/817543/All_pk_domains/3'>This link</scene> will highlight each domain of the protein.
Clicking <scene name='81/817543/All_pk_domains/3'>here</scene> will highlight each domain of Parkin by color.


Parkin is a monomeric 465-residues long protein containing an <span style="color:green">'''Ubiquitin-like (Ubl)'''</span> domain, a <span style="color:blue">'''RING0'''</span> domain, a <span style="color:purple">'''RING1'''</span> domain, an <span style="color:black">'''In Between Rings (IBR)'''</span> domain, a <span style="color:red">'''repressor (REP)'''</span> element and a <span style="color:brown">'''RING2'''</span> domain. Parkin is produced by the cell in an autoinhibited state. The <scene name='81/818543/Ubl/1'>Ubl domain</scene> maintains a compact <scene name='81/817543/Ring0-ring1/2'>RING0-RING1 interface</scene>, and together with the REP element, , prevents the E2 from binding to the RING1 domain. Phosphorylation of the <scene name='81/817543/Ser65_pk/3'>Ser65 residue</scene> in the Ubl domain leads to a change in conformation in the tertiary structure of the protein in the RING0-RING1 interface, which is optimized for pUb binding. However, both pUb and pUbl cannot be bound to parkin at the same time, which is consistent with the proposed allosteric loss of structure to the C-terminus of Helix H3 and IBR domain that would interfere with the Ubl-binding site.
Parkin is a monomeric 465-residues long protein containing an <span style="color:green">'''Ubiquitin-like (Ubl)'''</span> domain, a <span style="color:blue">'''RING0'''</span> domain, a <span style="color:purple">'''RING1'''</span> domain, an <span style="color:black">'''In Between Rings (IBR)'''</span> domain, a <span style="color:red">'''repressor (REP)'''</span> element and a <span style="color:brown">'''RING2'''</span> domain. Parkin is produced by the cell in an autoinhibited state. The <scene name='81/818543/Ubl/1'>Ubl domain</scene> maintains a compact <scene name='81/817543/Ring0-ring1/2'>RING0-RING1 interface</scene>, and together with the REP element, , prevents the E2 from binding to the RING1 domain. Phosphorylation of the <scene name='81/817543/Ser65_pk/3'>Ser65 residue</scene> in the Ubl domain leads to a change in conformation in the tertiary structure of the protein in the RING0-RING1 interface, which is optimized for pUb binding. However, both pUb and pUbl cannot be bound to parkin at the same time, which is consistent with the proposed allosteric loss of structure to the C-terminus of Helix H3 and IBR domain that would interfere with the Ubl-binding site.
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