4csp

Structure of the F306C mutant of nitrite reductase from Achromobacter xylosoxidans

spinqualitylabelsall models 4csp, resolution 1.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4csp is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CU, MES, ZN Related: 4csz Activity: Nitrite reductase (NO-forming), with EC number 1.7.2.1 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Enzyme mechanisms are often probed by structure-informed point mutations and measurement of their effects on enzymatic properties to test mechanistic hypotheses. In many cases, the challenge is to report on complex, often inter-linked elements of catalysis. Evidence for long-range effects on enzyme mechanism resulting from mutations remains sparse, limiting the design/redesign of synthetic catalysts in a predictable way. Here we show that improving the accessibility of the active site pocket of copper nitrite reductase by mutation of a surface-exposed phenylalanine residue (Phe306), located 12 A away from the catalytic site type-2 Cu (T2Cu), profoundly affects intra-molecular electron transfer, substrate-binding and catalytic activity. Structures and kinetic studies provide an explanation for the lower affinity for the substrate and the alteration of the rate-limiting step in the reaction. Our results demonstrate that distant residues remote from the active site can have marked effects on enzyme catalysis, by driving mechanistic change through relatively minor structural perturbations. Impact of residues remote from the catalytic centre on enzyme catalysis of copper nitrite reductase.,Leferink NG, Antonyuk SV, Houwman JA, Scrutton NS, Eady RR, Hasnain SS Nat Commun. 2014 Jul 15;5:4395. doi: 10.1038/ncomms5395. PMID:25022223 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Nitrite reductase

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