1kh0

Accurate Computer Base Design of a New Backbone Conformation in the Second Turn of Protein L

spinqualitylabelsall models 1kh0, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1kh0 is a 2 chain structure with sequence from Finm2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 1hz5, 1hz6 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The rational design of loops and turns is a key step towards creating proteins with new functions. We used a computational design procedure to create new backbone conformations in the second turn of protein L. The Protein Data Bank was searched for alternative turn conformations, and sequences optimal for these turns in the context of protein L were identified using a Monte Carlo search procedure and an energy function that favors close packing. Two variants containing 12 and 14 mutations were found to be as stable as wild-type protein L. The crystal structure of one of the variants has been solved at a resolution of 1.9 A, and the backbone conformation in the second turn is remarkably close to that of the in silico model (1.1 A RMSD) while it differs significantly from that of wild-type protein L (the turn residues are displaced by an average of 7.2 A). The folding rates of the redesigned proteins are greater than that of the wild-type protein and in contrast to wild-type protein L the second beta-turn appears to be formed at the rate limiting step in folding. Accurate computer-based design of a new backbone conformation in the second turn of protein L.,Kuhlman B, O'Neill JW, Kim DE, Zhang KY, Baker D J Mol Biol. 2002 Jan 18;315(3):471-7. PMID:11786026 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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