6jhy: Difference between revisions
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==Crystal Structure of the S1 subunit N-terminal domain from DcCoV UAE-HKU23 spike protein== | |||
<StructureSection load='6jhy' size='340' side='right'caption='[[6jhy]], [[Resolution|resolution]] 2.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6jhy]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JHY FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jhy OCA], [http://pdbe.org/6jhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jhy RCSB], [http://www.ebi.ac.uk/pdbsum/6jhy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jhy ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The DcCoV UAE-HKU23 coronavirus is a newly-found betacoronavirus (betaCoV) that can infect human cells. The viral spike protein plays pivotal roles in mediating receptor-recognition and membrane-fusion, and is therefore a key factor involved in viral pathogenesis and inter-species transmission. Here we reported the structural and functional characterization of the spike N-terminal domain (NTD) from DcCoV UAE-HKU23 (HKU23-NTD). Via mucin-binding assays, we showed that HKU23-NTD is able to bind sugars. We further solved the structure of HKU23-NTD, performed structure-guided mutagenesis and successfully located the potential sugar-binding pockets in the structure. Furthermore, via comparison of available betaCoV NTD structures, we demonstrated that betaCoV NTDs contain a conserved beta-sandwich core, but exhibit variant folds in the peripheral elements located in the top-ceiling region and on the lateral side. While showing different compositions and structures, these peripheral elements are topologically equivalent beta-sandwich-core insertions, highlighting a divergent evolution process for betaCoVs to form different lineages. | |||
Crystal structure of the S1 subunit N-terminal domain from DcCoV UAE-HKU23 spike protein.,Cheng Y, He B, Yang J, Ye F, Lin S, Yang F, Chen Z, Chen Z, Cao Y, Lu G Virology. 2019 Jun 27;535:74-82. doi: 10.1016/j.virol.2019.06.015. PMID:31279241<ref>PMID:31279241</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6jhy" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Cheng, Y]] | |||
[[Category: Lu, G]] | |||
[[Category: Ye, F]] | |||
[[Category: Crystal structure]] | |||
[[Category: Dccov uae-hku23]] | |||
[[Category: Viral protein]] | |||
Revision as of 06:13, 24 July 2019
Crystal Structure of the S1 subunit N-terminal domain from DcCoV UAE-HKU23 spike protein
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