6oqp: Difference between revisions
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==U-AITx-Ate1== | |||
<StructureSection load='6oqp' size='340' side='right'caption='[[6oqp]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6oqp]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OQP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OQP FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6oqp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oqp OCA], [http://pdbe.org/6oqp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oqp RCSB], [http://www.ebi.ac.uk/pdbsum/6oqp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oqp ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Sea anemone venoms have long been recognised as a rich source of peptides with interesting pharmacological and structural properties. Our recent transcriptomic studies of the Australian sea anemone Actinia tenebrosa have identified a novel 13-residue peptide, U-AITx-Ate1. U-AITx-Ate1 contains a single disulfide bridge and bears no significant homology to previously reported amino acid sequences of peptides from sea anemones or other species. We have produced U-AITx-Ate1 using solid-phase peptide synthesis, followed by oxidative folding and purification of the folded peptide using reversed-phase high-performance liquid chromatography. The solution structure of U-AITx-Ate1 was determined based on two-dimensional nuclear magnetic resonance spectroscopic data. Diffusion-ordered NMR spectroscopy revealed that U-AITx-Ate1 was monomeric in solution. Perturbations in the 1D (1)H NMR spectrum of U-AITx-Ate1 in the presence of dodecylphosphocholine micelles together with molecular dynamics simulations indicated an interaction of U-AITx-Ate1 with lipid membranes, although no binding was detected to 100% POPC and 80% POPC: 20% POPG lipid nanodiscs by isothermal titration calorimetry. Functional assays were performed to explore the biological activity profile of U-AITx-Ate1. U-AITx-Ate1 showed no activity in voltage-clamp electrophysiology assays and no change in behaviour and mortality rates in crustacea. Moderate cytotoxic activity was observed against two breast cancer cell lines. | |||
Structural and functional characterisation of a novel peptide from the Australian sea anemone Actinia tenebrosa.,Elnahriry KA, Wai DCC, Krishnarjuna B, Badawy NN, Chittoor B, MacRaild CA, Williams-Noonan BJ, Surm JM, Chalmers DK, Zhang AH, Peigneur S, Mobli M, Tytgat J, Prentis P, Norton RS Toxicon. 2019 Jul 11;168:104-112. doi: 10.1016/j.toxicon.2019.07.002. PMID:31302115<ref>PMID:31302115</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6oqp" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Elnahriry, K A]] | |||
[[Category: Norton, R S]] | |||
[[Category: Wai, D C.C]] | |||
[[Category: Antitumor protein]] | |||
[[Category: Sea anemone cysteine-containing peptide structure nmr spectroscopy lipid interaction]] | |||
