6ren: Difference between revisions
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==Crystal structure of 3fPizza6-SH with Zn2+== | |||
<StructureSection load='6ren' size='340' side='right'caption='[[6ren]], [[Resolution|resolution]] 1.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6ren]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6REN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6REN FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ren FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ren OCA], [http://pdbe.org/6ren PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ren RCSB], [http://www.ebi.ac.uk/pdbsum/6ren PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ren ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We developed an artificial hydrolase based on the symmetrical Pizza6 beta-propeller protein for the metal-free hydrolysis of 4-nitrophenyl acetate and butyrate. Through site-specific mutagenesis and crystallisation studies, the catalytic mechanism was investigated and found to be dependent on a threonine-histidine dyad. The mutant with additional histidine residues generated the highest kcat values, forming a His-His-Thr triad and matched previously reported metalloenzymes. The highly symmetrical beta-propeller artificial enzymes and their protein-metal complexes have potential to be utilised in bioinorganic and supramolecular chemistry, as well as being developed further into 2D/3D catalytic materials. | |||
Artificial beta-propeller protein-based hydrolases.,Clarke DE, Noguchi H, Gryspeerdt JAG, De Feyter S, Voet ARD Chem Commun (Camb). 2019 Jul 23;55(60):8880-8883. doi: 10.1039/c9cc04388h. PMID:31321399<ref>PMID:31321399</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6ren" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Clarke, D E]] | |||
[[Category: Feyter, S D]] | |||
[[Category: Gryspeerdt, J L]] | |||
[[Category: Noguchi, H]] | [[Category: Noguchi, H]] | ||
[[Category: | [[Category: Voet, A R.D]] | ||
[[Category: | [[Category: Artificial beta-propeller protein]] | ||
[[Category: | [[Category: Computational design]] | ||
[[Category: | [[Category: De novo protein]] | ||
[[Category: Metalloprotein]] | |||
[[Category: Pizza]] | |||
[[Category: Zinc]] | |||
Revision as of 06:33, 31 July 2019
Crystal structure of 3fPizza6-SH with Zn2+
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