6q4v

KlenTaq DNA polymerase in complex with dATP

spinqualitylabelsall models 6q4v, resolution 2.01Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6q4v is a 3 chain structure with sequence from Atcc 25104. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: DTP, EDO, MG, MN NonStd Res: DOC Gene: polA, pol1 (ATCC 25104) Activity: DNA-directed DNA polymerase, with EC number 2.7.7.7 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Archaeal B-family DNA polymerases (DNA pols) are the driving force of cutting edge biotechnological applications like next-generation sequencing. The acceptance of chemically modified nucleotides by the DNA pols is key to these technologies. By now, no structural data are available for these DNA pols in complex with modified substrates which could build the basis for understanding interactions between the enzyme and the chemical modification and for further development of next generation nucleotides. For the first time we crystallized an exonuclease deficient variant of B-family KOD DNA pol wild-type with a modified nucleotide in a closed, ternary complex. We also crystalized the A-family DNA pol KlenTaq with the same nucleotide. The reported structural data elucidates how the protein and the DNA modulate two distinct conformations of the modification in the A- and B-family DNA pols and how these influence the processing of the modified nucleotide. Overall, this study provides first insights on how B-family DNA pols interplay with relevant modified substrates. Structure of an archaeal B-family DNA polymerase in complex with a chemically modified nucleotide.,Marx A, Kropp H, Diederichs K Angew Chem Int Ed Engl. 2019 Feb 14. doi: 10.1002/anie.201900315. PMID:30761722 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also DNA polymerase 3D structures

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