6can: Difference between revisions
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==Prolyl oligopeptidase mutant S477C from Pyrococcus furiosus== | ==Prolyl oligopeptidase mutant S477C from Pyrococcus furiosus== | ||
<StructureSection load='6can' size='340' side='right' caption='[[6can]], [[Resolution|resolution]] 2.20Å' scene=''> | <StructureSection load='6can' size='340' side='right'caption='[[6can]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6can]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CAN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CAN FirstGlance]. <br> | <table><tr><td colspan='2'>[[6can]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CAN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CAN FirstGlance]. <br> | ||
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6can FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6can OCA], [http://pdbe.org/6can PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6can RCSB], [http://www.ebi.ac.uk/pdbsum/6can PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6can ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6can FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6can OCA], [http://pdbe.org/6can PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6can RCSB], [http://www.ebi.ac.uk/pdbsum/6can PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6can ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Enzymes in the prolyl oligopeptidase family possess unique structures and substrate specificities that are important for their biological activity and for potential biocatalytic applications. The crystal structures of Pyrococcus furiosus ( Pfu) prolyl oligopeptidase (POP) and the corresponding S477C mutant were determined to 1.9 and 2.2 A resolution, respectively. The wild type enzyme crystallized in an open conformation, indicating that this state is readily accessible, and it contained bound chloride ions and a prolylproline ligand. These structures were used as starting points for molecular dynamics simulations of Pfu POP conformational dynamics. The simulations showed that large-scale domain opening and closing occurred spontaneously, providing facile substrate access to the active site. Movement of the loop containing the catalytically essential histidine into a conformation similar to those found in structures with fully formed catalytic triads also occurred. This movement was modulated by chloride binding, providing a rationale for experimentally observed activation of POP peptidase catalysis by chloride. Thus, the structures and simulations reported in this study, combined with existing biochemical data, provide a number of insights into POP catalysis. | |||
Crystal Structure and Conformational Dynamics of Pyrococcus furiosus Prolyl Oligopeptidase.,Ellis-Guardiola K, Rui H, Beckner RL, Srivastava P, Sukumar N, Roux B, Lewis JC Biochemistry. 2019 Mar 26;58(12):1616-1626. doi: 10.1021/acs.biochem.9b00031., Epub 2019 Mar 5. PMID:30786206<ref>PMID:30786206</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6can" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Atcc 43587]] | [[Category: Atcc 43587]] | ||
[[Category: Large Structures]] | |||
[[Category: Ellis-Guardiola, K]] | [[Category: Ellis-Guardiola, K]] | ||
[[Category: Lewis, J C]] | [[Category: Lewis, J C]] | ||
Revision as of 06:26, 21 August 2019
Prolyl oligopeptidase mutant S477C from Pyrococcus furiosus
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