6i2z: Difference between revisions
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The | ==Isolated globin domain of the Bordetella pertussis globin-coupled sensor== | ||
<StructureSection load='6i2z' size='340' side='right'caption='[[6i2z]], [[Resolution|resolution]] 3.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6i2z]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I2Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I2Z FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4uiq|4uiq]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6i2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i2z OCA], [http://pdbe.org/6i2z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i2z RCSB], [http://www.ebi.ac.uk/pdbsum/6i2z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i2z ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
AIMS: Structural and functional characterization of the globin coupled sensors (GCS) of <i>Azotobacter vinelandii </i>(AvGReg) and <i>Bordetella pertussis</i> (BpeGReg). RESULTS: UV/Vis and RR spectroscopies confirm the presence in <i>Av</i>GReg and <i>Bpe</i>GReg of a globin domain capable of reversible gaseous ligand binding. In <i>Av</i>GReg an influence of the transmitter domain on the heme proximal region of the globin domain can be seen, and <i>k'</i>CO is higher than for other GCSs. The O<sub>2</sub> binding kinetics suggest the presence of an open and a closed conformation. As for <i>Bpe</i>GReg, the fully oxygenated <i>Av</i>GReg show a very high DGC activity. The CO rebinding to <i>Bpe</i>GReg indicates that intra- and intermolecular interactions influence the ligand binding. The globin domains of both proteins (<i>Av</i>GReg-Gb and <i>Bpe</i>Greg-Gb*) share the same GCS fold, a similar proximal but a different distal side structure. They homodimerize through a G-H helical bundle as in other GCS. However, <i>Bpe</i>GReg-Gb* shows also a second dimerization mode. INNOVATION: This paper extends our knowledge on the GCS proteins and contributes to a better understanding of the GCSs' role in the formation of bacterial biofilms. CONCLUSIONS: <i>Av</i>GReg and <i>Bpe</i>Greg conform to the GCS family, share a similar overall structure but they have different properties in terms of the ligand binding. In particular, <i>Av</i>Greg shows an open and closed conformation that in the latter form will very tightly bind oxygen. <i>Bpe</i>GReg has only one closed conformation. In both proteins, it is the fully oxygenated GCS form that catalyzes the production of the second messenger. | |||
Structural and functional characterization of the globin-coupled sensors of <i>Azotobacter vinelandii </i>and <i>Bordetella pertussis</i>.,Germani F, Nardini M, De Schutter A, Cuypers B, Berghmans H, Van Hauwaert ML, Stefano B, Mozzarelli A, Moens L, Van Doorslaer S, Bolognesi M, Pesce A, Dewilde S Antioxid Redox Signal. 2019 Sep 27. doi: 10.1089/ars.2018.7690. PMID:31559835<ref>PMID:31559835</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6i2z" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Berghmans, H]] | [[Category: Berghmans, H]] | ||
[[Category: Bolognesi, M]] | |||
[[Category: Bruno, S]] | [[Category: Bruno, S]] | ||
[[Category: Cuypers, B]] | [[Category: Cuypers, B]] | ||
[[Category: Dewilde, S]] | [[Category: Dewilde, S]] | ||
[[Category: | [[Category: Doorslaer, S Van]] | ||
[[Category: | [[Category: Germani, F]] | ||
[[Category: Hauwaert, M L.Van]] | |||
[[Category: Moens, L]] | [[Category: Moens, L]] | ||
[[Category: Mozzarelli, A]] | |||
[[Category: Pesce, A]] | |||
[[Category: Schutter, A De]] | |||
[[Category: Biofilm formation]] | |||
[[Category: C-di-gmp and enzyme specificity]] | |||
[[Category: Heme-based sensor]] | |||
[[Category: Oxygen affinity]] | |||
[[Category: Transferase]] | |||
