6et8

Crystal structure of AlbA in complex with albicidin

spinqualitylabelsall models 6et8, resolution 1.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6et8 is a 2 chain structure with sequence from "bacillus_oxytocus_perniciosus"_flugge_1886 "bacillus oxytocus perniciosus" flugge 1886. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: BWH, SO4 NonStd Res: MSE Gene: albA ("Bacillus oxytocus perniciosus" Flugge 1886) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The worldwide emergence of antibiotic resistance poses a serious threat to human health. A molecular understanding of resistance strategies employed by bacteria is obligatory to generate less-susceptible antibiotics. Albicidin is a highly potent antibacterial compound synthesized by the plant-pathogenic bacterium Xanthomonas albilineans. The drug-binding protein AlbA confers albicidin resistance to Klebsiella oxytoca. Here we show that AlbA binds albicidin with low nanomolar affinity resulting in full inhibition of its antibacterial activity. We report on the crystal structure of the drug-binding domain of AlbA (AlbAS) in complex with albicidin. Both alpha-helical repeat domains of AlbAS are required to cooperatively clamp albicidin, which is unusual for drug-binding proteins of the MerR family. Structure-guided NMR binding studies employing synthetic albicidin derivatives give valuable information about ligand promiscuity of AlbAS. Our findings thus expand the general understanding of antibiotic resistance mechanisms and support current drug-design efforts directed at more effective albicidin analogs. Molecular insights into antibiotic resistance - how a binding protein traps albicidin.,Rostock L, Driller R, Gratz S, Kerwat D, von Eckardstein L, Petras D, Kunert M, Alings C, Schmitt FJ, Friedrich T, Wahl MC, Loll B, Mainz A, Sussmuth RD Nat Commun. 2018 Aug 6;9(1):3095. doi: 10.1038/s41467-018-05551-4. PMID:30082794 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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