6dwo: Difference between revisions
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==Crystal structure of alpha-1-2-mannosidase from Enterococcus faecalis V583== | |||
<StructureSection load='6dwo' size='340' side='right'caption='[[6dwo]], [[Resolution|resolution]] 2.15Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6dwo]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DWO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DWO FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dwo OCA], [http://pdbe.org/6dwo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dwo RCSB], [http://www.ebi.ac.uk/pdbsum/6dwo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dwo ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
While multiple alpha 1-2-mannosidases are necessary for glycoprotein N-glycan maturation in vertebrates, a single bacterial alpha1-2-mannosidase can be sufficient to cleave all alpha1-2-linked mannose residues in host glycoprotein N-glycans. We report here the characterization and crystal structure of a new alpha1-2-mannosidase (EfMan-I) from Enterococcus faecalis, a Gram-positive opportunistic human pathogen. EfMan-I catalyzes the cleavage of alpha1-2-mannose from not only oligomannoses but also high-mannose-type N-glycans on glycoproteins. Its 2.15 A resolution crystal structure reveals a two-domain enzyme fold similar to other CAZy GH92 mannosidases. An unexpected potassium ion was observed bridging two domains near the active site. These findings support EfMan-I as an effective catalyst for in vitro N-glycan modification of glycoproteins with high-mannose-type N-glycans. | |||
Enterococcus faecalis alpha1-2-mannosidase (EfMan-I): an efficient catalyst for glycoprotein N-glycan modification.,Li Y, Li R, Yu H, Sheng X, Wang J, Fisher AJ, Chen X FEBS Lett. 2019 Sep 25. doi: 10.1002/1873-3468.13618. PMID:31552675<ref>PMID:31552675</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Fisher, A | <div class="pdbe-citations 6dwo" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Fisher, A J]] | |||
[[Category: Li, Y]] | [[Category: Li, Y]] | ||
[[Category: Enterococcus faecali]] | |||
[[Category: Hydrolase]] | |||
[[Category: Mannosidase]] | |||
Revision as of 08:57, 30 October 2019
Crystal structure of alpha-1-2-mannosidase from Enterococcus faecalis V583
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