5w94: Difference between revisions

Revision as of 16:59, 20 November 2019

Crystal structure of Scc4 in complex with Scc2n and Ctf19n

Structural highlights

5w94 is a 6 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:	SEP, TPO
Related:	4xdn
Gene:	SCC4, YER147C (ATCC 18824), SCC2, YDR180W, YD9395.14 (ATCC 18824)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SCC4_YEAST] Involved in sister chromatid cohesion. Forms a complex with SCC2, which is required for the association of the cohesin complex with chromosomes.^[1] [SCC2_YEAST] Plays a structural role in chromatin. Involved in sister chromatid cohesion. Forms a complex with SCC4 required for the stable association of the cohesin complex with chromatin, which may act by hydrolyzing ATP from SMC1 and SMC3 heads. Also participates in DNA repair.^[2] ^[3]

Publication Abstract from PubMed

The ring-shaped cohesin complex brings together distant DNA domains to maintain, express, and segregate the genome. Establishing specific chromosomal linkages depends on cohesin recruitment to defined loci. One such locus is the budding yeast centromere, which is a paradigm for targeted cohesin loading. The kinetochore, a multiprotein complex that connects centromeres to microtubules, drives the recruitment of high levels of cohesin to link sister chromatids together. We have exploited this system to determine the mechanism of specific cohesin recruitment. We show that phosphorylation of the Ctf19 kinetochore protein by a conserved kinase, DDK, provides a binding site for the Scc2/4 cohesin loading complex, thereby directing cohesin loading to centromeres. A similar mechanism targets cohesin to chromosomes in vertebrates. These findings represent a complete molecular description of targeted cohesin loading, a phenomenon with wide-ranging importance in chromosome segregation and, in multicellular organisms, transcription regulation.

The Kinetochore Receptor for the Cohesin Loading Complex.,Hinshaw SM, Makrantoni V, Harrison SC, Marston AL Cell. 2017 Sep 21;171(1):72-84.e13. doi: 10.1016/j.cell.2017.08.017. PMID:28938124^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ciosk R, Shirayama M, Shevchenko A, Tanaka T, Toth A, Shevchenko A, Nasmyth K. Cohesin's binding to chromosomes depends on a separate complex consisting of Scc2 and Scc4 proteins. Mol Cell. 2000 Feb;5(2):243-54. PMID:10882066
↑ Ciosk R, Shirayama M, Shevchenko A, Tanaka T, Toth A, Shevchenko A, Nasmyth K. Cohesin's binding to chromosomes depends on a separate complex consisting of Scc2 and Scc4 proteins. Mol Cell. 2000 Feb;5(2):243-54. PMID:10882066
↑ Arumugam P, Gruber S, Tanaka K, Haering CH, Mechtler K, Nasmyth K. ATP hydrolysis is required for cohesin's association with chromosomes. Curr Biol. 2003 Nov 11;13(22):1941-53. PMID:14614819
↑ Hinshaw SM, Makrantoni V, Harrison SC, Marston AL. The Kinetochore Receptor for the Cohesin Loading Complex. Cell. 2017 Sep 21;171(1):72-84.e13. doi: 10.1016/j.cell.2017.08.017. PMID:28938124 doi:https://dx.doi.org/10.1016/j.cell.2017.08.017

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OCA

[1] Ciosk R, Shirayama M, Shevchenko A, Tanaka T, Toth A, Shevchenko A, Nasmyth K. Cohesin's binding to chromosomes depends on a separate complex consisting of Scc2 and Scc4 proteins. Mol Cell. 2000 Feb;5(2):243-54. PMID:10882066

[2] Ciosk R, Shirayama M, Shevchenko A, Tanaka T, Toth A, Shevchenko A, Nasmyth K. Cohesin's binding to chromosomes depends on a separate complex consisting of Scc2 and Scc4 proteins. Mol Cell. 2000 Feb;5(2):243-54. PMID:10882066

[3] Arumugam P, Gruber S, Tanaka K, Haering CH, Mechtler K, Nasmyth K. ATP hydrolysis is required for cohesin's association with chromosomes. Curr Biol. 2003 Nov 11;13(22):1941-53. PMID:14614819

[4] Hinshaw SM, Makrantoni V, Harrison SC, Marston AL. The Kinetochore Receptor for the Cohesin Loading Complex. Cell. 2017 Sep 21;171(1):72-84.e13. doi: 10.1016/j.cell.2017.08.017. PMID:28938124 doi:https://dx.doi.org/10.1016/j.cell.2017.08.017

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@@ Line 1: / Line 1: @@
 ==Crystal structure of Scc4 in complex with Scc2n and Ctf19n==
-<StructureSection load='5w94' size='340' side='right' caption='[[5w94]], [[Resolution|resolution]] 3.19&Aring;' scene=''>
+<StructureSection load='5w94' size='340' side='right'caption='[[5w94]], [[Resolution|resolution]] 3.19&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5w94]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W94 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W94 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5w94]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W94 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W94 FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4xdn|4xdn]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SCC4, YER147C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), SCC2, YDR180W, YD9395.14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w94 OCA], [http://pdbe.org/5w94 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w94 RCSB], [http://www.ebi.ac.uk/pdbsum/5w94 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w94 ProSAT]</span></td></tr>
 </table>
@@ Line 23: / Line 24: @@
 __TOC__
 </StructureSection>
+[[Category: Atcc 18824]]
+[[Category: Large Structures]]
 [[Category: Harrison, S C]]
 [[Category: Hinshaw, S M]]

5w94: Difference between revisions

Revision as of 16:59, 20 November 2019

Crystal structure of Scc4 in complex with Scc2n and Ctf19n

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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