4y5f

PAS-GAF fragment from Deinococcus radiodurans BphP assembled with BV - Y307S, high dose

spinqualitylabelsall models 4y5f, resolution 1.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4y5f is a 1 chain structure with sequence from Deira. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: LBV Related: 4y3i Gene: bphP, DR_A0050 (DEIRA) Activity: Histidine kinase, with EC number 2.7.13.3 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [BPHY_DEIRA] Photoreceptor which exists in two forms that are reversibly interconvertible by light: the R form that absorbs maximally in the red region of the spectrum and the FR form that absorbs maximally in the far-red region. Has also a slight blue shift for the far-red maximum. Could also absorb green light. May participate in regulating pigment synthesis like the carotenoid deinoxanthin which could protect the bacterium from intense visible light. Publication Abstract from PubMed We report that in the red light-absorbing (Pr) state, the bilin chromophore of the Deinococcus radiodurans proteobacterial phytochrome (DrBphP) is hypersensitive to X-ray photons used in typical synchrotron X-ray protein crystallography experiments. This causes the otherwise fully protonated chromophore to deprotonate without additional major structural changes. These results have major implications for our understanding of the structural and chemical characteristics of the resting and intermediate states of phytochromes and other photoreceptor proteins. X-ray radiation induces deprotonation of the bilin chromophore in crystalline D. radiodurans phytochrome.,Li F, Burgie ES, Yu T, Heroux A, Schatz GC, Vierstra RD, Orville AM J Am Chem Soc. 2015 Mar 4;137(8):2792-5. doi: 10.1021/ja510923m. Epub 2015 Feb, 18. PMID:25650486 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA