194l: Difference between revisions
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'''THE 1.40 A STRUCTURE OF SPACEHAB-01 HEN EGG WHITE LYSOZYME''' | '''THE 1.40 A STRUCTURE OF SPACEHAB-01 HEN EGG WHITE LYSOZYME''' | ||
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[[Category: Ries-Kautt, M.]] | [[Category: Ries-Kautt, M.]] | ||
[[Category: Vaney, M C.]] | [[Category: Vaney, M C.]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:35:27 2008'' | |||
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Revision as of 06:35, 2 May 2008
THE 1.40 A STRUCTURE OF SPACEHAB-01 HEN EGG WHITE LYSOZYME
Overview
Crystals of tetragonal hen egg-white lysozyme were grown using Advanced Protein Crystallization Facility (APCF) apparatus under a microgravity environment (SpaceHab-01 mission) and ground control conditions. Crystals were grown from NaCl as a crystallizing agent at pH 4.3. The X-ray diffraction patterns of the best diffracting ground- and space-grown crystals were recorded using synchrotron radiation and an image plate on the W32 beamline at LURE. Both ground- and space-grown crystals showed nearly equivalent maximum resolution of 1.3-1.4 A. Refinements were carried out with the program X-PLOR with final R values of 18.45 and 18.27% for structures from ground- and space- grown crystals, respectively. The two structures are nearly identical with the root-mean-square difference on all protein atoms being 0.13 A. Some residues of the two refined structures show multiple alternative conformations. Two ions were localized into the electron-density maps of the two structures: one chloride ion at the interface between two symmetry-related molecules and one sodium ion stabilizing the loop Ser60-Leu75. The sodium ion is surrounded by six ligands which form a bipyramid around it at distances of 2.2-2.6 A.
About this Structure
194L is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
High-resolution structure (1.33 A) of a HEW lysozyme tetragonal crystal grown in the APCF apparatus. Data and structural comparison with a crystal grown under microgravity from SpaceHab-01 mission., Vaney MC, Maignan S, Ries-Kautt M, Ducriux A, Acta Crystallogr D Biol Crystallogr. 1996 May 1;52(Pt 3):505-17. PMID:15299672 Page seeded by OCA on Fri May 2 09:35:27 2008
