6bhn

Red Light-Absorbing State of NpR6012g4, a Red/Green Cyanobacteriochrome

spinqualitylabelsall models 6bhn, 10 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6bhn is a 1 chain structure with sequence from Nosp7. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CYC Related: 6bho Gene: Npun_R6012 (NOSP7) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Phytochrome photoreceptors control plant growth, development, and the shade avoidance response that limits crop yield in high-density agricultural plantings. Cyanobacteriochromes (CBCRs) are distantly related photosensory proteins that control cyanobacterial metabolism and behavior in response to light. Photoreceptors in both families reversibly photoconvert between two photostates via photoisomerization of linear tetrapyrrole (bilin) chromophores. Spectroscopic and biochemical studies have demonstrated heterogeneity in both photostates, but the structural basis for such heterogeneity remains unclear. We report solution NMR structures for both photostates of the red/green CBCR NpR6012g4 from Nostoc punctiforme In addition to identifying structural changes accompanying photoconversion, these structures reveal structural heterogeneity for residues Trp655 and Asp657 in the red-absorbing NpR6012g4 dark state, yielding two distinct environments for the phycocyanobilin chromophore. We use site-directed mutagenesis and fluorescence and absorbance spectroscopy to assign an orange-absorbing population in the NpR6012g4 dark state to the minority configuration for Asp657. This population does not undergo full, productive photoconversion, as shown by time-resolved spectroscopy and absorption spectroscopy at cryogenic temperature. Our studies thus elucidate the spectral and photochemical consequences of structural heterogeneity in a member of the phytochrome superfamily, insights that should inform efforts to improve photochemical or fluorescence quantum yields in the phytochrome superfamily. Correlating structural and photochemical heterogeneity in cyanobacteriochrome NpR6012g4.,Lim S, Yu Q, Gottlieb SM, Chang CW, Rockwell NC, Martin SS, Madsen D, Lagarias JC, Larsen DS, Ames JB Proc Natl Acad Sci U S A. 2018 Apr 9. pii: 1720682115. doi:, 10.1073/pnas.1720682115. PMID:29632180 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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