Sandbox reserved 1560

β

This Sandbox is Reserved from Aug 26 through Dec 12, 2019 for use in the course CHEM 351 Biochemistry taught by Bonnie_Hall at the Grand View University, Des Moines, USA. This reservation includes Sandbox Reserved 1556 through Sandbox Reserved 1575.

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Lignostilbene-α,β-dioxygenase A (LsdA) Catalyzation

spinqualitylabelsall models Crystal Structure of Sphingomonas paucimobilis apo-LsdA Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Lignostilbene-α,β-dioxygenase A (LsdA) is a catalytic enzyme found in bacteria to break down cellulose components. Function Lignostilbene-α,β-dioxygenase A (LsdA) is an enzyme found in the bacteria Sphingomonas paucimbilis. It is used to catalyze the substrate, lignostilbene, into two vanillin molecules. Vanillin is a member of the benzaldehydes class and plays a role in the plant metabolite. Lignin is found in the plant cell walls, lignocellulose, and it gives the plant its rigid structure. The depolymerization of the lignin can allow for better use of the plants resources as a source of biofuel. Lignostilbene The lignostilbene is a compound found in cellulose Vanillin Vanillin is the product from the cleavage of lignostilbene from LsdA. Cleavage of Lignostilbene Broader Implications Lignocellulosic biomasses have been used for biofuels, mainly bio-ethanol. The abundant avaiability of the raw material make the use of biomasses ideal. With the rise of carbon emission, biomasses can be used to substitue the need for carbon based fuel, as long as, the biomass is carbon neutral . Lignostilbene is the second most abundant raw material. Energy Transformation When Phe 59 is substituted with His and Tyr 101 is substituted with Phe, the k (app/cat) decreases in activity by 15-10 folds. The Lys 134 substitution of Met inhibits the enzyme activity. Phenylazophenol inhibits the LsdA-catalyzed cleavage of lignostilbene with a competitive and uncompetitive inhibition. Structural highlights catalytic triad and spacefill seven-bladed β-propeller There are ligands in each Chain. Lignostilbene is a quationary structure with two identical proteins working together. The proteins are partially composed of alpha helix(Blue) and the majority by Beta-sheets indicated in red.