1a4k: Difference between revisions
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{{STRUCTURE_1a4k| PDB=1a4k | SCENE= }} | |||
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'''DIELS ALDER CATALYTIC ANTIBODY WITH TRANSITION STATE ANALOGUE''' | '''DIELS ALDER CATALYTIC ANTIBODY WITH TRANSITION STATE ANALOGUE''' | ||
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[[Category: Spiller, B W.]] | [[Category: Spiller, B W.]] | ||
[[Category: Stevens, R C.]] | [[Category: Stevens, R C.]] | ||
[[Category: | [[Category: Antibody]] | ||
[[Category: | [[Category: Catalytic antibody]] | ||
[[Category: | [[Category: Diels alder]] | ||
[[Category: | [[Category: Germline]] | ||
[[Category: | [[Category: Immunoglobulin]] | ||
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Revision as of 06:48, 2 May 2008
DIELS ALDER CATALYTIC ANTIBODY WITH TRANSITION STATE ANALOGUE
Overview
The three-dimensional structure of an antibody (39-A11) that catalyzes a Diels-Alder reaction has been determined. The structure suggests that the antibody catalyzes this pericyclic reaction through a combination of packing and hydrogen-bonding interactions that control the relative geometries of the bound substrates and electronic distribution in the dienophile. A single somatic mutation, serine-91 of the light chain to valine, is largely responsible for the increase in affinity and catalytic activity of the affinity-matured antibody. Structural and functional studies of the germ-line precursor suggest that 39-A11 and related antibodies derive from a family of germ-line genes that have been selected throughout evolution for the ability of the encoded proteins to form a polyspecific combining site. Germ line-encoded antibodies of this type, which can rapidly evolve into high-affinity receptors for a broad range of structures, may help to expand the binding potential associated with the structural diversity of the primary antibody repertoire.
About this Structure
1A4K is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Immunological origins of binding and catalysis in a Diels-Alderase antibody., Romesberg FE, Spiller B, Schultz PG, Stevens RC, Science. 1998 Mar 20;279(5358):1929-33. PMID:9506942 Page seeded by OCA on Fri May 2 09:48:13 2008
