4xwf

Crystal structure of the ZMP riboswitch at 1.80 angstrom

spinqualitylabelsall models 4xwf, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4xwf is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: AMZ, CAC, IRI, MG Related: 4xw7 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The ZTP riboswitch is a widespread family of regulatory RNAs that upregulate de novo purine synthesis in response to increased intracellular levels of ZTP or ZMP. As an important intermediate in purine biosynthesis, ZMP also serves as a proxy for the concentration of N10-formyl-tetrahydrofolate, a key component of one-carbon metabolism. Here, we report the structure of the ZTP riboswitch bound to ZMP at a resolution of 1.80 A. The RNA contains two subdomains brought together through a long-range pseudoknot further stabilized through helix-helix packing. ZMP is bound at the subdomain interface of the RNA through a set of interactions with the base, ribose sugar, and phosphate moieties of the ligand. Unique to nucleobase recognition by RNAs, the Z base is inner-sphere coordinated to a magnesium cation bound by two backbone phosphates. This interaction, along with steric hindrance by the backbone, imparts specificity over chemically similar compounds such as ATP/AMP. Metal Ion-Mediated Nucleobase Recognition by the ZTP Riboswitch.,Trausch JJ, Marcano-Velazquez JG, Matyjasik MM, Batey RT Chem Biol. 2015 Jul 23;22(7):829-37. doi: 10.1016/j.chembiol.2015.06.007. Epub, 2015 Jul 2. PMID:26144884 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Riboswitch

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