5cqg

Structure of Tribolium telomerase in complex with the highly specific inhibitor BIBR1532

spinqualitylabelsall models 5cqg, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5cqg is a 2 chain structure with sequence from Red flour beetle. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: 55C Gene: TERT, TcasGA2_TC010963 (Red flour beetle) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed BIBR1532 is a highly specific telomerase inhibitor, although the molecular basis for inhibition is unknown. Here we present the crystal structure of BIBR1532 bound to Tribolium castaneum catalytic subunit of telomerase (tcTERT). BIBR1532 binds to a conserved hydrophobic pocket (FVYL motif) on the outer surface of the thumb domain. The FVYL motif is near TRBD residues that bind the activation domain (CR4/5) of hTER. RNA binding assays show that the human TERT (hTERT) thumb domain binds the P6.1 stem loop of CR4/5 in vitro. hTERT mutations of the FVYL pocket alter wild-type CR4/5 binding and cause telomere attrition in cells. Furthermore, the hTERT FVYL mutations V1025F, N1028H, and V1090M are implicated in dyskeratosis congenita and aplastic anemia, further supporting the biological and clinical relevance of this novel motif. We propose that CR4/5 contacts with the telomerase thumb domain contribute to telomerase ribonucleoprotein assembly and promote enzymatic activity. Structural Basis of Telomerase Inhibition by the Highly Specific BIBR1532.,Bryan C, Rice C, Hoffman H, Harkisheimer M, Sweeney M, Skordalakes E Structure. 2015 Oct 6;23(10):1934-1942. doi: 10.1016/j.str.2015.08.006. Epub 2015, Sep 10. PMID:26365799 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Telomerase

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