5hhj

Reverse transcriptase domain of group II intron maturase from Roseburia intestinalis in P21 space group

spinqualitylabelsall models 5hhj, resolution 1.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5hhj is a 2 chain structure with sequence from Roseburia intestinalis xb6b4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GLY, K, MPD, PO4, SIN Related: 5hhk, 5hhl Gene: RO1_37670 (Roseburia intestinalis XB6B4) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Group II introns are self-splicing ribozymes that are essential in many organisms, and they have been hypothesized to share a common evolutionary ancestor with the spliceosome. Although structural similarity of RNA components supports this connection, it is of interest to determine whether associated protein factors also share an evolutionary heritage. Here we present the crystal structures of reverse transcriptase (RT) domains from two group II intron-encoded proteins (maturases) from Roseburia intestinalis and Eubacterium rectale, obtained at 1.2-A and 2.1-A resolution, respectively. These domains are more similar in architecture to the spliceosomal Prp8 RT-like domain than to any other RTs, and they share substantial similarity with flaviviral RNA polymerases. The RT domain itself is sufficient for binding intron RNA with high affinity and specificity, and it is contained within an active RT enzyme. These studies provide a foundation for understanding structure-function relationships within group II intron-maturase complexes. Crystal structures of a group II intron maturase reveal a missing link in spliceosome evolution.,Zhao C, Pyle AM Nat Struct Mol Biol. 2016 May 2. doi: 10.1038/nsmb.3224. PMID:27136328 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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