6mpz: Difference between revisions
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==Crystal structure of a double glycine motif protease from AMS/PCAT transporter in complex with the leader peptide== | |||
<StructureSection load='6mpz' size='340' side='right'caption='[[6mpz]], [[Resolution|resolution]] 2.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6mpz]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Lachnospiraceae_bacterium_c6a11 Lachnospiraceae bacterium c6a11] and [http://en.wikipedia.org/wiki/Promm Promm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MPZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MPZ FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=16P:3,6,9,12,15,18-HEXAOXAICOSANE'>16P</scene></td></tr> | |||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLZ:AMINO-ACETALDEHYDE'>GLZ</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mpz OCA], [http://pdbe.org/6mpz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mpz RCSB], [http://www.ebi.ac.uk/pdbsum/6mpz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mpz ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The secretion of peptides and proteins is essential for survival and ecological adaptation of bacteria. Dual-functional ATP-binding cassette transporters export antimicrobial or quorum signaling peptides in Gram-positive bacteria. Their substrates contain a leader sequence that is excised by an N-terminal peptidase C39 domain at a double Gly motif. We characterized the protease domain (LahT150) of a transporter from a lanthipeptide biosynthetic operon in Lachnospiraceae and demonstrate that this protease can remove the leader peptide from a diverse set of peptides. The 2.0 A resolution crystal structure of the protease domain in complex with a covalently bound leader peptide demonstrates the basis for substrate recognition across the entire class of such transporters. The structural data also provide a model for understanding the role of leader peptide recognition in the translocation cycle, and the function of degenerate, non-functional C39-like domains (CLD) in substrate recruitment in toxin exporters in Gram-negative bacteria. | |||
Insights into AMS/PCAT transporters from biochemical and structural characterization of a double Glycine motif protease.,Bobeica SC, Dong SH, Huo L, Mazo N, McLaughlin MI, Jimenez-Oses G, Nair SK, van der Donk WA Elife. 2019 Jan 14;8. pii: 42305. doi: 10.7554/eLife.42305. PMID:30638446<ref>PMID:30638446</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Nair, S | <div class="pdbe-citations 6mpz" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Lachnospiraceae bacterium c6a11]] | |||
[[Category: Large Structures]] | |||
[[Category: Promm]] | |||
[[Category: Dong, S H]] | |||
[[Category: Nair, S K]] | |||
[[Category: Lantibiotic]] | |||
[[Category: Leader peptide]] | |||
[[Category: Peptidase c39 domain]] | |||
[[Category: Peptide secretion]] | |||
[[Category: Transport protein]] | |||
Revision as of 11:27, 1 January 2020
Crystal structure of a double glycine motif protease from AMS/PCAT transporter in complex with the leader peptide
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