6b12

Structure of Tne2 in complex with Tni2

spinqualitylabelsall models 6b12, resolution 1.71Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6b12 is a 4 chain structure with sequence from Psef5. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. NonStd Res: MSE Gene: PFL_6209 (PSEF5), PFL_6210 (PSEF5) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The bacterial type VI secretion system (T6SS) mediates antagonistic cell-cell interactions between competing Gram-negative bacteria. In plant-beneficial bacteria, this pathway has been shown to suppress the growth of bacterial pathogens; however, the identification and mode of action of T6SS effector proteins that mediate this protective effect remain poorly defined. Here, we identify two previously uncharacterized effectors required for interbacterial antagonism by the plant commensal bacterium Pseudomonas protegens. Consistent with the established effectorimmunity paradigm for antibacterial T6SS substrates, the toxic activities of these effectors are neutralized by adjacently encoded cognate immunity determinants. While one of these effectors, RhsA, belongs to family of DNase enzymes, the activity of the other was not apparent from its sequence. To determine the mechanism of toxicity of this latter effector, we determined its 1.3A crystal structure in complex with its immunity protein and found that it resembles NAD(P)(+) degrading enzymes. In line with this structural similarity, biochemical characterization of this effector, termed Tne2 (<span style="text-decoration: underline;">t</span>ype VI secretion <span style="text-decoration: underline;">N</span>ADase <span style="text-decoration: underline;">e</span>ffector family 2), demonstrates that it possesses potent NAD(P)(+) hydrolase activity. Tne2 is the founding member of a widespread family of interbacterial NADases predicted to transit not only the Gramnegative T6SS but also the Gram-positive type VII secretion system, a pathway recently implicated in interbacterial competition among Firmicutes. Together, this work identifies new T6SS effectors employed by a plant commensal bacterium to antagonize its competitors and broadly implicates NAD(P)(+) hydrolyzing enzymes as substrates of interbacterial conflict pathways found in diverse bacterial phyla. Diverse NADase effector families mediate interbacterial antagonism via the type VI secretion system.,Tang JY, Bullen NP, Ahmad S, Whitney JC J Biol Chem. 2017 Dec 13. pii: RA117.000178. doi: 10.1074/jbc.RA117.000178. PMID:29237732 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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