6du7

Glutathione reductase from Streptococcus pneumoniae

spinqualitylabelsall models 6du7, resolution 2.56Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6du7 is a 8 chain structure with sequence from "diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: FAD, SCN Gene: gor, A5N45_09080, ERS020408_00588, ERS022363_01056 ("Diplococcus pneumoniae" (Klein 1884) Weichselbaum 1886) Activity: Glutathione-disulfide reductase, with EC number 1.8.1.7 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The glutathione reductase (GR) from Streptococcus pneumoniae is a flavoenzyme that catalyzes the reduction of oxidized glutathione (GSSG) to its reduced form (GSH) in the cytoplasm of this bacterium. The maintenance of an intracellular pool of GSH is critical for the detoxification of reactive oxygen and nitrogen species and for intracellular metal tolerance to ions such as zinc. Here, S. pneumoniae GR (SpGR) was overexpressed and purified and its crystal structure determined at 2.56 A resolution. SpGR shows overall structural similarity to other characterized GRs, with a dimeric structure that includes an antiparallel beta-sheet at the dimer interface. This observation, in conjunction with comparisons with the interface structures of other GR enzymes, allows the classification of these enzymes into three classes. Analyses of the kinetic properties of SpGR revealed a significantly higher value for Km(GSSG) (231.2 +/- 24.7 microM) in comparison to other characterized GR enzymes. The structure and activity of the glutathione reductase from Streptococcus pneumoniae.,Sikanyika M, Aragao D, McDevitt CA, Maher MJ Acta Crystallogr F Struct Biol Commun. 2019 Jan 1;75(Pt 1):54-61. doi:, 10.1107/S2053230X18016527. Epub 2019 Jan 1. PMID:30605126 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Glutathione Reductase

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