Sandbox Reserved 1101: Difference between revisions
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'''5IZ2 : Crystal structure of the ''N. Clavipes'' spidroin NTD''' | '''5IZ2 : Crystal structure of the ''N. Clavipes'' spidroin NTD''' | ||
5IZ2 is the NTD domain of a protein called spidroin. This protein is a component of the dragline silk. | 5IZ2 is the NTD domain of a protein called spidroin[https://en.wikipedia.org/wiki/Spidroin]. This protein is a component of the dragline silk. | ||
There are several types of spidroin, and those that form the core of the silk are called '''MaSp1''' (Major ampullate Spidroin-1), which are produced by in the major ampullate gland of spiders. | There are several types of spidroin, and those that form the core of the silk are called '''MaSp1''' (Major ampullate Spidroin-1), which are produced by in the major ampullate gland of spiders. | ||
The NTD domain of these proteins is very important because it plays a major role in the dimerisation of spidroins. Indeed, thanks to the NTD organization, 2 spidroins can be combined, leading to the production of fibres with exceptional physical qualities. | The NTD domain of these proteins is very important because it plays a major role in the dimerisation of spidroins. Indeed, thanks to the NTD organization, 2 spidroins can be combined, leading to the production of fibres with exceptional physical qualities. | ||
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== Monomer structure of the spidroin NTD domain == | == Monomer structure of the spidroin NTD domain == | ||
One monomer of NTD is composed of 2 subunits, A and B, each of them composed of 5 α-helices. There is also a chain Z composed of 3 amino acids (Ser, Tyr, Gly), but it role is not well established yet. | |||
In each subunit, the orientation of helices 2, 3 and 5 is different from the orientation of helices 1 and 4. Indeed, helices 1 and 4 form the rigid body of the NTD domain, while helices 2, 3 and 5 are involved in intermolecular contacts, so they play an important role in the dimerization process. | |||
Moreover, at the opposite extremities of each subunits of the monomer there are clusters of acidic residus (Asp36, Asp39, Asp40, Glu79, Asp91) in one part, and clusters of basic residus (Lys54, Arg57, Lys60, Lys64, Lys65) in the other part. So, this create a dipole moment. And, as the subunits A and B are organized antiparallel, it allows an access to charges poles. | |||
== Dimerization of the spidroin by the NTD domain == | == Dimerization of the spidroin by the NTD domain == | ||
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This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. | This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. | ||
== Applications in Biotechnology == | |||
</StructureSection> | </StructureSection> | ||
== References == | == References == | ||
<references/> | <references/> | ||
