Sandbox Reserved 1091: Difference between revisions

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A peptide can be inserted in the space of the active site. There, the amino acids of the catalytic triad will interact together and the mechanism will lead to a cut in the polypeptide.  
A peptide can be inserted in the space of the active site. There, the amino acids of the catalytic triad will interact together and the mechanism will lead to a cut in the polypeptide.  


/Users/Amandine/Desktop/Capture d’écran 2019-12-27 à 22.20.04.png
'''Mechanism:''' The mechanism is the following: The histidine will react with the serine and deprotonate it. '''The deprotonated hydroxyl group of the serine will act as a nucleophilic species''' and attack the carbon from the carbonyl function on the peptide. This will lead to the formation of a tetrahedral intermediate. Then, a second tetrahedral intermediate will be formed, but with the attack of a deprotonated water molecule. At the end, the regeneration of the active site will be done with the release of the peptide cut in two parts.  


'''Mechanism:''' The mechanism is the following: The histidine will react with the serine and deprotonate it. '''The deprotonated hydroxyl group of the serine will act as a nucleophilic species''' and attack the carbon from the carbonyl function on the peptide. This will lead to the formation of a tetrahedral intermediate. Then, a second tetrahedral intermediate will be formed, but with the attack of a deprotonated water molecule. At the end, the regeneration of the active site will be done with the release of the peptide cut in two parts.
  https://upload.wikimedia.org/wikipedia/commons/1/17/Serine_protease_mechanism_by_snellios.png
  https://upload.wikimedia.org/wikipedia/commons/1/17/Serine_protease_mechanism_by_snellios.png


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